catalog number :
MBS917434
products type :
ELISA Kit
products full name :
Dog Hepcidin, HAMP ELISA Kit
products short name :
hepcidin antimicrobial peptide
products name syn :
Dog Hepcidin (HAMP) ELISA kit; HEPC; HEPCIDIN; HFE2B; LEAP-1; LEAP1; PLTR; liver-expressed antimicrobial peptide; putative liver tumor regressor; hepcidin antimicrobial peptide
other names :
hepcidin; Hepcidin; hepcidin
products gene name :
HAMP
other gene names :
HAMP; HAMP
uniprot entry name :
HEPC_CANFA
specificity :
This assay has high sensitivity and excellent specificity for detection of dog HAMP. No significant cross-reactivity or interference between dog HAMP and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: 0.78 ng/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HAMP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HAMP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HAMP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HAMP bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001007141.1
ncbi gb acc num :
NM_001007140.1
ncbi mol weight :
9,172 Da
uniprot summary :
Function: Seems to act as a signaling molecule involved in the maintenance of iron homeostasis. Seems to be required in conjunction with HFE to regulate both intestinal iron absorption and iron storage in macrophages. May also have antimicrobial activity
size4 :
10x96-Strip-Wells