Rat Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit | MyBioSource MBS917167 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rat Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

catalog :

MBS917167

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS917167

products type :

ELISA Kit

products full name :

Rat Prostaglandin-H2 D-isomerase, PTGDS ELISA Kit

products short name :

prostaglandin D2 synthase 21kDa (brain)

products name syn :

Rat Prostaglandin-H2 D-isomerase (PTGDS) ELISA kit; RP11-229P13.6; L-PGDS; LPGDS; PDS; PGD2; PGDS; PGDS2; PGD2 synthase; beta-trace protein; glutathione-independent PGD synthase; lipocalin-type prostaglandin D synthase; prostaglandin D synthase; prostagla; prostaglandin D2 synthase 21kDa (brain)

other names :

prostaglandin-H2 D-isomerase; Prostaglandin-H2 D-isomerase; prostaglandin-H2 D-isomerase; PGDS; PGDS2; PGD2 synthase; Prostaglandin D synthase; prostaglandin-D2 synthase; glutathione-independent PGD synthase; glutathione-independent PGD synthetase; lipocalin-type prostaglandin-D synthase; prostaglandin D2 synthase (brain); Glutathione-independent PGD synthase; Lipocalin-type prostaglandin-D synthase; Prostaglandin-D2 synthase; PGD2 synthase; PGDS; PGDS2

products gene name :

PTGDS

other gene names :

Ptgds; Ptgds; PH2DISO; PGD2 synthase; PGDS; PGDS2

uniprot entry name :

PTGDS_RAT

reactivity :

Rat

sequence length :

189

specificity :

This assay has high sensitivity and excellent specificity for detection of rat PTGDS. No significant cross-reactivity or interference between rat PTGDS and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cerebrospinal fluid (CSF), tissue homogenates. Assay Type: Sandwich. Detection Range: 9.37 ng/ml -600 ng/ml. Sensitivity: 2.34 ng /ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PTGDS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PTGDS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PTGDS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PTGDS bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

6981430

ncbi acc num :

NP_037147.1

ncbi gb acc num :

NM_013015.2

uniprot acc num :

P22057

ncbi mol weight :

21,301 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (83383); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198497); Folic Acid Network Pathway (219799); Prostaglandin Synthesis And Regulation Pathway (198437); Selenium Pathway (219762)

ncbi summary :

catalyzes conversion of prostaglandin H2 to prostaglandin D2, a major prostaglandin that mediates sleep, body temperature, hormone release, and odor responses [RGD, Feb 2006]

uniprot summary :

Function: Catalyzes the conversion of PGH2 to PGD2, a prostaglandin involved in smooth muscle contraction/relaxation and a potent inhibitor of platelet aggregation. Involved in a variety of CNS functions, such as sedation, NREM sleep and PGE2-induced allodynia, and may have an anti-apoptotic role in oligodendrocytes. Binds small non-substrate lipophilic molecules, including biliverdin, bilirubin, retinal, retinoic acid and thyroid hormone, and may act as a scavenger for harmful hydrophopic molecules and as a secretory retinoid and thyroid hormone transporter. Possibly involved in development and maintenance of the blood-brain, blood-retina, blood-aqueous humor and blood-testis barrier. It is likely to play important roles in both maturation and maintenance of the central nervous system and male reproductive system.4 PublicationsManual assertion based on experiment in:Ref.8. Catalytic activity: (5Z,13E,15S)-9-alpha,11-alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E,15S)-9-alpha,15-dihydroxy-11-oxoprosta-5,13-dienoate.

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage