ELISA/assay

Human Sterol regulatory element-binding protein 1, SREBF1 ELISA Kit

MBS916923

48-Strip-Wells

510 USD

ELISA Kit

Human Sterol regulatory element-binding protein 1, SREBF1 ELISA Kit

sterol regulatory element binding transcription factor 1

Human Sterol regulatory element-binding protein 1 (SREBF1) ELISA kit; SREBP-1c; SREBP1; bHLHd1; sterol regulatory element binding protein-1; sterol regulatory element binding transcription factor 1

sterol regulatory element-binding protein 1 isoform a; Sterol regulatory element-binding protein 1; sterol regulatory element-binding protein 1; SREBP-1; class D basic helix-loop-helix protein 1; sterol regulatory element binding transcription factor 1; Class D basic helix-loop-helix protein 1; bHLHd1; Sterol regulatory element-binding transcription factor 1Cleaved into the following chain:Processed sterol regulatory element-binding protein 1

SREBF1

SREBF1; SREBF1; SREBP1; bHLHd1; SREBP-1c; BHLHD1; SREBP1; SREBP-1; bHLHd1

SRBP1_HUMAN

Human

1177

This assay has high sensitivity and excellent specificity for detection of human SREBF1. No significant cross-reactivity or interference between human SREBF1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml -500 pg/ml. Sensitivity: 1.95 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SREBF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SREBF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SREBF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SREBF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

52630419

NP_001005291.1

NM_001005291.2

P36956

121,675 Da

Adipogenesis Pathway (198832); Caspase Cascade In Apoptosis Pathway (137974); Circadian Clock Pathway (187173); Developmental Biology Pathway (477129); Fatty Acid, Triacylglycerol, And Ketone Body Metabolism Pathway (160977); Id Signaling Pathway (198871); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976)

This gene encodes a transcription factor that binds to the sterol regulatory element-1 (SRE1), which is a decamer flanking the low density lipoprotein receptor gene and some genes involved in sterol biosynthesis. The protein is synthesized as a precursor that is attached to the nuclear membrane and endoplasmic reticulum. Following cleavage, the mature protein translocates to the nucleus and activates transcription by binding to the SRE1. Sterols inhibit the cleavage of the precursor, and the mature nuclear form is rapidly catabolized, thereby reducing transcription. The protein is a member of the basic helix-loop-helix-leucine zipper (bHLH-Zip) transcription factor family. This gene is located within the Smith-Magenis syndrome region on chromosome 17. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

SREBP-1: Transcriptional activator required for lipid homeostasis. Regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. Binds to the sterol regulatory element 1 (SRE-1) (5 -ATCACCCCAC-3 ). Has dual sequence specificity binding to both an E-box motif (5 -ATCACGTGA-3 ) and to SRE-1 (5 -ATCACCCCAC-3 ). Forms a tight complex with SCAP in the ER membrane. Efficient DNA binding of the soluble transcription factor fragment requires dimerization with another bHLH protein. Interacts with LMNA. Expressed in a wide variety of tissues, most abundant in liver and adrenal gland. In fetal tissues lung and liver shows highest expression. Isoform SREBP-1C predominates in liver, adrenal gland and ovary, whereas isoform SREBP-1A predominates in hepatoma cell lines. Isoform SREBP-1A and isoform SREBP-1C are found in kidney, brain, white fat, and muscle. Belongs to the SREBP family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral; Membrane protein, multi-pass; DNA-binding; Transcription factor. Chromosomal Location of Human Ortholog: 17p11.2. Cellular Component: Golgi membrane; nucleoplasm; endoplasmic reticulum membrane; protein complex; endoplasmic reticulum; cytoplasm; integral to membrane; nuclear envelope; nucleus; cytosol. Molecular Function: protein dimerization activity; protein binding; sterol response element binding; DNA binding; sequence-specific DNA binding; protein complex binding; chromatin binding; transcription factor activity; protein kinase binding. Biological Process: circadian rhythm; response to cAMP; response to glucagon stimulus; lipid biosynthetic process; positive regulation of cholesterol biosynthetic process; negative regulation of transcription from RNA polymerase II promoter; cellular lipid metabolic process; negative regulation of insulin secretion; positive regulation of histone deacetylation; regulation of fatty acid metabolic process; response to glucose stimulus; aging; response to drug; response to food; cholesterol metabolic process; response to retinoic acid; transcription, DNA-dependent; cellular response to starvation; regulation of transcription from RNA polymerase II promoter; chemical signal regulation of heart rate; insulin receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; lipid metabolic process; response to progesterone stimulus; lung development

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800