ELISA/assay

Mouse Regenerating islet-derived protein 3-alpha, REG3A ELISA Kit

MBS916893

48-Strip-Wells

510 USD

ELISA Kit

Mouse Regenerating islet-derived protein 3-alpha, REG3A ELISA Kit

regenerating islet-derived 3 alpha

Mouse Regenerating islet-derived protein 3-alpha (REG3A) ELISA kit; HIP; PAP; PAP-H; PAP1; PBCGF; REG-III; REG3; PAP homologous protein; hepatocarcinoma-intestine-pancreas; pancreatic beta cell growth factor; pancreatitis-associated protein; proliferation-inducing prote; regenerating islet-derived 3 alpha

regenerating islet-derived protein 3-alpha; Regenerating islet-derived protein 3-alpha; regenerating islet-derived protein 3-alpha; REG-3-alpha; reg III-alpha; RegIII (alpha); lithostathine 3; pancreatitis-associated protein 2; islet of Langerhans regenerating protein 3; regenerating islet-derived protein III-alpha; generating islet-derived, mouse homolog 3 alpha; regenerating islet-derived 3 alpha; Islet of Langerhans regenerating protein 3; Lithostathine 3; Pancreatitis-associated protein 2; Regenerating islet-derived protein III-alpha; Reg III-alpha

REG3A

Reg3a; Reg3a; AV063448; Pap2; REG-3-alpha; Reg III-alpha

REG3A_MOUSE

Mouse

175

This assay has high sensitivity and excellent specificity for detection of mouse REG3A. No significant cross-reactivity or interference between mouse REG3A and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of mouse REG3A is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for REG3A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any REG3A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for REG3A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of REG3A bound in the initial step. The color development is stopped and the intensity of the color is measured.

6755308

NP_035389.1

NM_011259.1

O09037

19,539 Da

REG3A: Might be a stress protein involved in the control of bacterial proliferation. Protein type: Secreted; Secreted, signal peptide. Cellular Component: extracellular region. Molecular Function: carbohydrate binding. Biological Process: negative regulation of keratinocyte differentiation; acute-phase response; inflammatory response

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800