product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Mouse Semaphorin-3F, SEMA3F ELISA Kit
catalog :
MBS916704
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS916704
products type :
ELISA Kit
products full name :
Mouse Semaphorin-3F, SEMA3F ELISA Kit
products short name :
sema domain, immunoglobulin domain (Ig) , short basic domain, secreted, (semaphorin) 3F
products name syn :
Mouse Semaphorin-3F (SEMA3F) ELISA kit; SEMA-IV; SEMA4; SEMAK; sema IV; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; 3F; semaphorin 3F; semaphorin III/F; semaphorin IV; sema domain; immunoglobulin domain (Ig); short basic domain; secreted; (semaphorin) 3F
other names :
semaphorin-3F; Semaphorin-3F; semaphorin-3F; semaphorin IV; sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3F; Semaphorin IV; Sema IV
products gene name :
SEMA3F
other gene names :
Sema3f; Sema3f; Sema4; Semak; Sema IV
uniprot entry name :
SEM3F_MOUSE
reactivity :
Mouse
sequence length :
754
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse SEMA3F. No significant cross-reactivity or interference between mouse SEMA3F and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0. 312 ng/ml -20 ng/ml. Sensitivity: The minimum detectable dose of mouse SEMA3F is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SEMA3F has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SEMA3F present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SEMA3F is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SEMA3F bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
31543683
ncbi acc num :
NP_035479.2
ncbi gb acc num :
NP_035479.2
uniprot acc num :
O88632
ncbi mol weight :
88,549 Da
ncbi pathways :
Axon Guidance Pathway (83262); Axon Guidance Pathway (476)
uniprot summary :
SEMA3F: May play a role in cell motility and cell adhesion. Belongs to the semaphorin family. Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis. Cellular Component: membrane; extracellular region. Molecular Function: chemorepellent activity; receptor activity. Biological Process: axon extension involved in axon guidance; trigeminal nerve structural organization; axon guidance; facial nerve structural organization; branchiomotor neuron axon guidance; multicellular organismal development; negative chemotaxis; nerve development; negative regulation of axon extension involved in axon guidance; neural crest cell migration
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

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