ELISA/assay

Human Pericentriolar material 1 protein, PCM1 ELISA Kit

MBS916674

96 Strip Wells

760 USD

ELISA Kit

Human Pericentriolar material 1 protein, PCM1 ELISA Kit

pericentriolar material 1

Human Pericentriolar material 1 protein (PCM1) ELISA kit; PTC4; ; pericentriolar material 1

pericentriolar material 1 protein; Pericentriolar material 1 protein; pericentriolar material 1 protein; PCM-1; hPCM-1; pericentriolar material 1, PCM1; pericentriolar material 1

PCM1

PCM1; PCM1; PTC4; PCM-1; hPCM-1

PCM1_HUMAN

Human

2024

This assay has high sensitivity and excellent specificity for detection of human PCM1. No significant cross-reactivity or interference between human PCM1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human pericentriolar material 1 protein (PCM1) concentrations in serum, plasma, tissue homogenates, cell lysates. Detection Range: 15.6 pg/ml -1000 pg/ml.

Sensitivity: The minimum detectable dose of human PCM1 is typically less than 3.9 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PCM1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCM1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCM1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCM1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

134142826

NP_006188.3

NM_006197.3

Q15154

228,533 Da

Cell Cycle Pathway 530733!!Cell Cycle, Mitotic Pathway 105765!!Centrosome Maturation Pathway 105807!!G2/M Transition Pathway 105801!!Loss Of Nlp From Mitotic Centrosomes Pathway 105811!!Loss Of Proteins Required For Interphase Microtubule Organization From The Centrosome Pathway 105810!!Mitotic G2-G2/M Phases Pathway 160942!!Recruitment Of Mitotic Centrosome Proteins And Complexes Pathway 105808

The protein encoded by this gene is a component of centriolar satellites, which are electron dense granules scattered around centrosomes. Inhibition studies show that this protein is essential for the correct localization of several centrosomal proteins, and for anchoring microtubules to the centrosome. Chromosomal aberrations involving this gene are associated with papillary thyroid carcinomas and a variety of hematological malignancies, including atypical chronic myeloid leukemia and T-cell lymphoma. [provided by RefSeq, Sep 2010]

Function: Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Involved in the biogenesis of cilia.5 PublicationsManual assertion based on experiment in:Ref.7

96 Strip Wells

760 USD