Human Ficolin-2, FCN2 ELISA Kit | MyBioSource MBS916649 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Ficolin-2, FCN2 ELISA Kit

catalog :

MBS916649

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS916649

products type :

ELISA Kit

products full name :

Human Ficolin-2, FCN2 ELISA Kit

products short name :

ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)

products name syn :

Human Ficolin-2 (FCN2) ELISA kit; RP11-263F14.2; EBP-37; FCNL; P35; ficolin-2; L-ficolin; collagen/fibrinogen domain-containing protein 2; ficolin 2; ficolin B; serum lectin p35; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin)

other names :

ficolin-2 isoform a; Ficolin-2; ficolin-2; L-ficolin; ficolin B; ficolin-B; ficolin-beta; serum lectin p35; 37 kDa elastin-binding protein; collagen/fibrinogen domain-containing protein 2; ficolin (collagen/fibrinogen domain containing lectin) 2 (hucolin); ficolin (collagen/fibrinogen domain containing lectin) 2; 37 kDa elastin-binding protein; Collagen/fibrinogen domain-containing protein 2; EBP-37; Ficolin-B; Ficolin-beta; Hucolin; L-ficolin; Serum lectin p35

products gene name :

FCN2

other gene names :

FCN2; FCN2; P35; FCNL; EBP-37; ficolin-2; FCNL

uniprot entry name :

FCN2_HUMAN

reactivity :

Human

sequence length :

313

specificity :

This assay has high sensitivity and excellent specificity for detection of human FCN2. No significant cross-reactivity or interference between human FCN2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.25 ng/ml -16 ng/ml. Sensitivity: The minimum detectable dose of human FCN2 is typically less than 0.06 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FCN2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FCN2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FCN2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FCN2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

61744445

ncbi acc num :

NP_004099.2

ncbi gb acc num :

NM_004108.2

uniprot acc num :

Q15485

ncbi mol weight :

34,001 Da

ncbi summary :

The product of this gene belongs to the ficolin family of proteins. This family is characterized by the presence of a leader peptide, a short N-terminal segment, followed by a collagen-like region, and a C-terminal fibrinogen-like domain. This gene is predominantly expressed in the liver, and has been shown to have carbohydrate binding and opsonic activities. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

FCN2: May function in innate immunity through activation of the lectin complement pathway. Calcium-dependent and GlcNAc- binding lectin. Enhances phagocytosis of S.typhimurium by neutrophils, suggesting an opsonic effect via the collagen region. Belongs to the ficolin lectin family. 2 isoforms of the human protein are produced by alternative splicing. Chromosomal Location of Human Ortholog: 9q34

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage