catalog number :
MBS916606
products type :
ELISA Kit
products full name :
Rat Mitochondrial brown fat uncoupling protein 1, UCP1 ELISA Kit
products short name :
uncoupling protein 1 (mitochondrial, proton carrier)
products name syn :
Rat Mitochondrial brown fat uncoupling protein 1 (UCP1) ELISA kit; SLC25A7; UCP; mitochondrial brown fat uncoupling protein; thermogenin; uncoupling protein 1; uncoupling protein 1 (mitochondrial; proton carrier)
other names :
mitochondrial brown fat uncoupling protein 1; Mitochondrial brown fat uncoupling protein 1; mitochondrial brown fat uncoupling protein 1; UCP 1; thermogenin; solute carrier family 25 member 7; Solute carrier family 25 member 7; Thermogenin
products gene name :
UCP1
other gene names :
UCP1; UCP1; SLC25A7; UCP; UCP 1
uniprot entry name :
UCP1_RABIT
specificity :
This assay has high sensitivity and excellent specificity for detection of rat UCP1. No significant cross-reactivity or interference between rat UCP1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Quantitative Sandwich. Sensitivity: Typically less than 7.81 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001164548.1
ncbi gb acc num :
NM_001171077.1
ncbi mol weight :
33,083 Da
uniprot summary :
Function: UCP are mitochondrial transporter proteins that create proton leaks across the inner mitochondrial membrane, thus uncoupling oxidative phosphorylation from ATP synthesis. As a result, energy is dissipated in the form of heat.
size4 :
10x96-Strip-Wells