ELISA/assay

Mouse Triggering receptor expressed on myeloid cells 2, TREM2 ELISA Kit

MBS916554

48-Strip-Wells

510 USD

ELISA Kit

Mouse Triggering receptor expressed on myeloid cells 2, TREM2 ELISA Kit

triggering receptor expressed on myeloid cells 2

Mouse Triggering receptor expressed on myeloid cells 2 (TREM2) ELISA kit; TREM-2; Trem2a; Trem2b; Trem2c; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2

triggering receptor expressed on myeloid cells 2 isoform 2; Triggering receptor expressed on myeloid cells 2; triggering receptor expressed on myeloid cells 2; triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2a; triggering receptor expressed on myeloid cells 2b; triggering receptor expressed on myeloid cells 2c; triggering receptor expressed on myeloid cells 2; Triggering receptor expressed on monocytes 2

TREM2

Trem2; Trem2; TREM-2; Trem2a; Trem2b; Trem2c; Trem2a; Trem2b; Trem2c; TREM-2

TREM2_MOUSE

Mouse

249

This assay has high sensitivity and excellent specificity for detection of Mouse TREM2. No significant cross-reactivity or interference between Mouse TREM2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml-10 ng/ml. Sensitivity: 0.039 ng/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TREM2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TREM2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TREM2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TREM2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

440918700

NP_001259007.1

NM_001272078.1

Q99NH8

24,527 Da

Axon Guidance Pathway (575211); Developmental Biology Pathway (575210); Osteoclast Differentiation Pathway (193149); Osteoclast Differentiation Pathway (193096); Other Semaphorin Interactions Pathway (575219); Semaphorin Interactions Pathway (575212)

The protein encoded by this gene is part of the immunoglobulin and lectin-like superfamily and functions as part of the innate immune system. This gene forms part of a cluster of genes on mouse chromosome 17 thought to be involved in innate immunity. This protein associates with the adaptor protein Dap-12 and recruits several factors, such as kinases and phospholipase C-gamma, to form a receptor signaling complex that activates myeloid cells, including dendritic cells and microglia. In humans homozygous loss-of-function mutations in this gene cause Nasu-Hakola disease and mutations in this gene may be risk factors to the development of Alzheimer's disease. In mouse mutations of this gene serve as a pathophysiological model for polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (Nasu-Hakola disease) and for inflammatory bowel disease. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jan 2013]

TREM2: May have a role in chronic inflammations and may stimulate production of constitutive rather than inflammatory chemokines and cytokines. Forms a receptor signaling complex with TYROBP and triggers activation of the immune responses in macrophages and dendritic cells. Defects in TREM2 are a cause of polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy (PLOSL); also known as presenile dementia with bone cysts or Nasu-Hakola disease (NHD). PLOSL is a recessively inherited disease characterized by a combination of psychotic symptoms rapidly progressing to presenile dementia and bone cysts restricted to wrists and ankles. PLOSL has a global distribution, although most of the patients have been diagnosed in Finland and Japan, with an estimated population prevalence of 2x10(-6) in the Finns. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Cellular Component: membrane; intracellular membrane-bound organelle; integral to membrane; extracellular region; plasma membrane. Molecular Function: peptidoglycan binding; protein binding; transmembrane receptor activity; lipopolysaccharide binding. Biological Process: positive regulation of peptidyl-tyrosine phosphorylation; detection of lipopolysaccharide; lipopolysaccharide-mediated signaling pathway; detection of peptidoglycan; innate immune response; positive regulation of calcium-mediated signaling; signal transduction; positive regulation of antigen processing and presentation of peptide antigen via MHC class II

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800