Human ATP-sensitive inward rectifier potassium channel 10, KCNJ10 ELISA Kit | MyBioSource MBS916440 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human ATP-sensitive inward rectifier potassium channel 10, KCNJ10 ELISA Kit

catalog :

MBS916440

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS916440

products type :

ELISA Kit

products full name :

Human ATP-sensitive inward rectifier potassium channel 10, KCNJ10 ELISA Kit

products short name :

potassium inwardly-rectifying channel, subfamily J, member 10

products name syn :

Human ATP-sensitive inward rectifier potassium channel 10 (KCNJ10) ELISA kit; BIRK-10; KCNJ13-PEN; KIR1.2; KIR4.1; SESAME; ATP-sensitive inward rectifier potassium channel 10; glial ATP-dependent inwardly rectifying potassium channel KIR4.1; inward rectifier K+ channel KIR1.2; i; potassium inwardly-rectifying channel; subfamily J; member 10

other names :

ATP-sensitive inward rectifier potassium channel 10; ATP-sensitive inward rectifier potassium channel 10; ATP-sensitive inward rectifier potassium channel 10; inward rectifier K+ channel KIR1.2; inward rectifier K(+) channel Kir1.2; ATP-dependent inwardly rectifying potassium channel Kir4.1; potassium channel, inwardly rectifying subfamily J member 10; glial ATP-dependent inwardly rectifying potassium channel KIR4.1; potassium inwardly-rectifying channel, subfamily J, member 10; ATP-dependent inwardly rectifying potassium channel Kir4.1; Inward rectifier K(+) channel Kir1.2; Potassium channel, inwardly rectifying subfamily J member 10

products gene name :

KCNJ10

other gene names :

KCNJ10; KCNJ10; KIR1.2; KIR4.1; SESAME; BIRK-10; KCNJ13-PEN

uniprot entry name :

KCJ10_HUMAN

reactivity :

Human

sequence length :

379

specificity :

This assay has high sensitivity and excellent specificity for detection of human KCNJ10. No significant cross-reactivity or interference between human KCNJ10 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 28 pg/ml -1800 pg/ml. Sensitivity: The minimum detectable dose of human KCNJ10 is typically less than 7 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for KCNJ10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any KCNJ10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for KCNJ10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of KCNJ10 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

25121966

ncbi acc num :

NP_002232.2

ncbi gb acc num :

NM_002241.4

uniprot acc num :

P78508

ncbi mol weight :

42,508 Da

ncbi pathways :

Activation Of G Protein Gated Potassium Channels Pathway (366226); Activation Of GABAB Receptors Pathway (187181); G Protein Gated Potassium Channels Pathway (366225); GABA B Receptor Activation Pathway (187180); GABA Receptor Activation Pathway (187178); Gastric Acid Secretion Pathway (154409); Gastric Acid Secretion Pathway (154383); Inhibition Of Voltage Gated Ca2+ Channels Via Gbeta/gamma Subunits Pathway (187183); Inwardly Rectifying K+ Channels Pathway (366224); Neuronal System Pathway (106513)

ncbi summary :

This gene encodes a member of the inward rectifier-type potassium channel family, characterized by having a greater tendency to allow potassium to flow into, rather than out of, a cell. The encoded protein may form a heterodimer with another potassium channel protein and may be responsible for the potassium buffering action of glial cells in the brain. Mutations in this gene have been associated with seizure susceptibility of common idiopathic generalized epilepsy syndromes. [provided by RefSeq, Jul 2008]

uniprot summary :

Kir4.1: May be responsible for potassium buffering action of glial cells in the brain. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by extracellular barium and cesium. Defects in KCNJ10 are the cause of seizures, sensorineural deafness, ataxia, mental retardation, and electrolyte imbalance (SESAMES). A complex disorder characterized by generalized seizures with onset in infancy, delayed psychomotor development, ataxia, sensorineural hearing loss, hypokalemia, metabolic alkalosis, and hypomagnesemia. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ10 subfamily. Protein type: Membrane protein, multi-pass; Channel, ligand-gated; Channel, potassium; Membrane protein, integral. Chromosomal Location of Human Ortholog: 1q23.2. Cellular Component: microvillus; basolateral plasma membrane; integral to plasma membrane; apical plasma membrane; plasma membrane. Molecular Function: identical protein binding; protein binding; ATP-activated inward rectifier potassium channel activity; ATP binding; receptor binding. Biological Process: regulation of long-term neuronal synaptic plasticity; myelination in the central nervous system; response to glucocorticoid stimulus; membrane hyperpolarization; response to blue light; glutamate uptake during transmission of nerve impulse; response to mineralocorticoid stimulus; adult walking behavior; protein homotetramerization; synaptic transmission; visual perception; potassium ion import; optic nerve development; regulation of resting membrane potential; regulation of sensory perception of pain; inflammatory response; potassium ion homeostasis; potassium ion transport. Disease: Pendred Syndrome; Seizures, Sensorineural Deafness, Ataxia, Mental Retardation, And Electrolyte Imbalance; Deafness, Autosomal Recessive 4, With Enlarged Vestibular Aqueduct

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage