Human Phosphatidylinositol-glycan-specific phospholipase D, GPLD1 ELISA Kit | MyBioSource MBS916407 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Phosphatidylinositol-glycan-specific phospholipase D, GPLD1 ELISA Kit

catalog :

MBS916407

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS916407

products type :

ELISA Kit

products full name :

Human Phosphatidylinositol-glycan-specific phospholipase D, GPLD1 ELISA Kit

products short name :

glycosylphosphatidylinositol specific phospholipase D1

products name syn :

Human Phosphatidylinositol-glycan-specific phospholipase D (GPLD1) ELISA kit; GPIPLD; GPIPLDM; MGC22590; PIGPLD; PIGPLD1; GPI-specific phospholipase D; glycoprotein phospholipase D; glycosylphosphatIdylinositol-specific phospholipase D; glycosylphosphatidylinositol specific phos; glycosylphosphatidylinositol specific phospholipase D1

other names :

phosphatidylinositol-glycan-specific phospholipase D; Phosphatidylinositol-glycan-specific phospholipase D; phosphatidylinositol-glycan-specific phospholipase D; GPI-PLD; PI-G PLD; GPI-specific phospholipase D; glycoprotein phospholipase D; glycosylphosphatidylinositol specific phospholipase D1, isoform 2; glycosylphosphatidylinositol specific phospholipase D1; Glycoprotein phospholipase D; Glycosyl-phosphatidylinositol-specific phospholipase D; GPI-PLD; GPI-specific phospholipase D

products gene name :

GPLD1

other gene names :

GPLD1; GPLD1; PLD; GPIPLD; PIGPLD; GPIPLDM; PIGPLD1; PIGPLD1; PI-G PLD; GPI-PLD; GPI-specific phospholipase D

uniprot entry name :

PHLD_HUMAN

reactivity :

Human

sequence length :

840

specificity :

This assay has high sensitivity and excellent specificity for detection of human GPLD1. No significant cross-reactivity or interference between human GPLD1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml -200 ng/ml. Sensitivity: The minimum detectable dose of human GPLD1 is typically less than 0.78 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GPLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GPLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GPLD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GPLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

29171717

ncbi acc num :

NP_001494.2

ncbi gb acc num :

NM_001503.3

uniprot acc num :

P80108

ncbi mol weight :

92,336 Da

ncbi pathways :

Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway (82988); Glycosylphosphatidylinositol(GPI)-anchor Biosynthesis Pathway (363)

ncbi summary :

Many proteins are tethered to the extracellular face of eukaryotic plasma membranes by a glycosylphosphatidylinositol (GPI) anchor. The GPI-anchor is a glycolipid found on many blood cells. The protein encoded by this gene is a GPI degrading enzyme. Glycosylphosphatidylinositol specific phospholipase D1 hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans, thereby releasing the attached protein from the plasma membrane. [provided by RefSeq, Jul 2008]

uniprot summary :

GPLD1: This protein hydrolyzes the inositol phosphate linkage in proteins anchored by phosphatidylinositol glycans (GPI-anchor) thus releasing these proteins from the membrane. Belongs to the GPLD1 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Glycan Metabolism - glycosylphosphatidylinositol (GPI)-anchor biosynthesis; Secreted, signal peptide; Phospholipase; EC 3.1.4.50; Secreted. Chromosomal Location of Human Ortholog: 6p22.1. Cellular Component: extracellular space; proteinaceous extracellular matrix; intracellular membrane-bound organelle; lysosomal membrane; cytoplasm; intracellular. Molecular Function: sodium channel regulator activity; glycosylphosphatidylinositol phospholipase D activity; phospholipase D activity. Biological Process: ossification; GPI anchor release; positive regulation of apoptosis; complement receptor mediated signaling pathway; positive regulation of membrane protein ectodomain proteolysis; phosphatidylcholine metabolic process; negative regulation of cell proliferation; cell migration during sprouting angiogenesis; cellular response to insulin stimulus; response to glucose stimulus; insulin receptor signaling pathway; positive regulation of secretion; chondrocyte differentiation; positive regulation of cytolysis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage