Human Ephrin-B2, EFNB2 ELISA Kit | MyBioSource MBS916324 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Ephrin-B2, EFNB2 ELISA Kit

catalog :

MBS916324

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS916324

products type :

ELISA Kit

products full name :

Human Ephrin-B2, EFNB2 ELISA Kit

products short name :

ephrin-B2

products name syn :

Human Ephrin-B2 (EFNB2) ELISA kit; RP11-272L14.1; EPLG5; HTKL; Htk-L; LERK5; MGC126226; MGC126227; MGC126228; HTK ligand; eph-related receptor tyrosine kinase ligand 5; ephrin B2; ligand of eph-related kinase 5; ephrin-B2

other names :

ephrin-B2; Ephrin-B2; ephrin-B2; LERK-5; HTK ligand; ligand of eph-related kinase 5; eph-related receptor tyrosine kinase ligand 5; ephrin-B2; EPH-related receptor tyrosine kinase ligand 5; LERK-5; HTK ligand; HTK-L

products gene name :

EFNB2

other gene names :

EFNB2; EFNB2; HTKL; EPLG5; Htk-L; LERK5; EPLG5; HTKL; LERK5; LERK-5; HTK-L

uniprot entry name :

EFNB2_HUMAN

reactivity :

Human

sequence length :

333

specificity :

This assay has high sensitivity and excellent specificity for detection of Human EFNB2. No significant cross-reactivity or interference between Human EFNB2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates and Cell lysates. Assay Type: Sandwich. Detection Range: 15.6 pg/ml-1000 pg/ml. Sensitivity: 3.9 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450nm. Sample Volume: 50-100ul. Protein Biological Process 1: Angiogenesis. Protein Biological Process 3: Angiogenesis

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EFNB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFNB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFNB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFNB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4758250

ncbi acc num :

NP_004084.1

ncbi gb acc num :

NM_004093.3

uniprot acc num :

P52799

ncbi mol weight :

36,923 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); EPHB Forward Signaling Pathway (138047); Ephrin B Reverse Signaling Pathway (138052); EphrinB-EPHB Pathway (137951)

ncbi summary :

This gene encodes a member of the ephrin (EPH) family. The ephrins and EPH-related receptors comprise the largest subfamily of receptor protein-tyrosine kinases and have been implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. This gene encodes an EFNB class ephrin which binds to the EPHB4 and EPHA3 receptors. [provided by RefSeq, Jul 2008]

uniprot summary :

EFNB2: a type I membrane protein of the ephrin family. A ligand of Eph-related receptor tyrosine kinases EphA3 and EphB4. Ephrins and ephrin receptors mediate numerous developmental processes, particularly in the nervous system. Ephrin-B2 is implicated in mediating developmental events, especially in the nervous system and in erythropoiesis. May play a role in constraining the orientation of longitudinally projecting axons. Protein type: Ligand, receptor tyrosine kinase; Membrane protein, integral. Chromosomal Location of Human Ortholog: 13q33. Cellular Component: focal adhesion; integral to plasma membrane; plasma membrane. Molecular Function: protein binding; ephrin receptor binding. Biological Process: axon guidance; anatomical structure morphogenesis; organ morphogenesis; regulation of chemotaxis; viral reproduction; cell-cell signaling; cell migration during sprouting angiogenesis; ephrin receptor signaling pathway; lymph vessel development; cell adhesion

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage