catalog number :
MBS916264
products type :
ELISA Kit
products full name :
Pig Cytochrome c, CYCS ELISA Kit
products short name :
cytochrome c, somatic
products name syn :
Pig Cytochrome c (CYCS) ELISA kit; CYC; HCS; THC4; OTTHUMP00000202043; OTTHUMP00000202044; cytochrome c; cytochrome c; somatic
other names :
cytochrome c; Cytochrome c; cytochrome c
products gene name :
CYCS
other gene names :
CYCS; CYCS; CYC; CYC
uniprot entry name :
CYC_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Pig CYCS. No significant cross-reactivity or interference between Pig CYCS and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 39 pg/ml-2500 pg/ml. Sensitivity: 9.75 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul. Protein Biological Process 1: Apoptosis/Autophagy. Protein Biological Process 3: Apoptosis
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYCS has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYCS present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYCS is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYCS bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001123442.1
ncbi gb acc num :
NM_001129970.1
ncbi mol weight :
11,704 Da
ncbi pathways :
Activation Of Caspases Through Apoptosome-mediated Cleavage Pathway (577962); Alzheimer's Disease Pathway (84508); Alzheimer's Disease Pathway (509); Amyotrophic Lateral Sclerosis (ALS) Pathway (84510); Amyotrophic Lateral Sclerosis (ALS) Pathway (511); Apoptosis Pathway (84472); Apoptosis Pathway (470); Apoptosis Pathway (577942); Apoptotic Factor-mediated Response Pathway (577959); Colorectal Cancer Pathway (84513)
uniprot summary :
CYCS: Plays a role in apoptosis. Suppression of the anti-apoptotic members or activation of the pro-apoptotic members of the Bcl-2 family leads to altered mitochondrial membrane permeability resulting in release of cytochrome c into the cytosol. Binding of cytochrome c to Apaf-1 triggers the activation of caspase-9, which then accelerates apoptosis by activating other caspases (By similarity). Protein type: Oxidoreductase; Protein phosphatase, Ser/Thr (non-receptor); Apoptosis; Mitochondrial
size4 :
10x96-Strip-Wells