Human Carboxypeptidase B, CPB1 ELISA Kit | MyBioSource MBS916209 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Carboxypeptidase B, CPB1 ELISA Kit

catalog :

MBS916209

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS916209

products type :

ELISA Kit

products full name :

Human Carboxypeptidase B, CPB1 ELISA Kit

products short name :

carboxypeptidase B1 (tissue)

products name syn :

Human Carboxypeptidase B (CPB1) ELISA kit; CPB; DKFZp779K1333; PASP; PCPB; pancreas-specific protein; pancreatic carboxypeptidase B1; procarboxypeptidase B; carboxypeptidase B1 (tissue)

other names :

carboxypeptidase B preproprotein; Carboxypeptidase B; carboxypeptidase B; protaminase; procarboxypeptidase B; pancreas-specific protein; tissue carboxypeptidase B; pancreatic carboxypeptidase B; carboxypeptidase B1 (tissue); Pancreas-specific protein; PASP

products gene name :

CPB1

other gene names :

CPB1; CPB1; CPB; PASP; PCPB; CPB; PCPB; PASP

uniprot entry name :

CBPB1_HUMAN

reactivity :

Human

sequence length :

417

specificity :

This assay has high sensitivity and excellent specificity for detection of human CPB1. No significant cross-reactivity or interference between human CPB1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 ng/ml -500 ng/ml. Sensitivity: The minimum detectable dose of human CPB1 is typically less than 1.95 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CPB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CPB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CPB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CPB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

54607080

ncbi acc num :

NP_001862.2

ncbi gb acc num :

NM_001871.2

uniprot acc num :

P15086

ncbi mol weight :

47,368 Da

ncbi pathways :

Pancreatic Secretion Pathway (169306); Pancreatic Secretion Pathway (169295); Protein Digestion And Absorption Pathway (172847); Protein Digestion And Absorption Pathway (171868)

ncbi summary :

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]

uniprot summary :

CPB1: Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]. Protein type: Secreted; EC 3.4.17.2; Secreted, signal peptide; Protease. Chromosomal Location of Human Ortholog: 3q24. Cellular Component: extracellular region. Molecular Function: carboxypeptidase activity; zinc ion binding; metallocarboxypeptidase activity. Biological Process: proteolysis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage