catalog number :
MBS915956
products type :
ELISA Kit
products full name :
Bovine Angiopoietin-1, ANGPT1 ELISA Kit
products short name :
angiopoietin 1
products name syn :
Bovine Angiopoietin-1 (ANGPT1) ELISA kit; AGP1; AGPT; ANG1; ; Bovine Angiopoietin-1 (ANGPT1)
other names :
angiopoietin-1; Angiopoietin-1; angiopoietin-1; ANG-1; angiopoietin 1<
products gene name :
ANGPT1
other gene names :
ANGPT1; ANGPT1; ANG1; ANG-1
uniprot entry name :
ANGP1_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Bovine ANGPT1. No significant cross-reactivity or interference between Bovine ANGPT1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm
other info2 :
Sample Volume: 50-100ul. Protein Biological Process 1: Cardiovascular
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ANGPT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ANGPT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ANGPT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ANGPT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001070265.1
ncbi gb acc num :
NM_001076797.1
ncbi mol weight :
57,429 Da
ncbi pathways :
Cell Surface Interactions At The Vascular Wall Pathway 581635!!Hemostasis Pathway 581607!!Rheumatoid Arthritis Pathway 200308!!Rheumatoid Arthritis Pathway 200269!!Tie2 Signaling Pathway 581636
uniprot summary :
Function: Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development