Human EGF-containing fibulin-like extracellular matrix protein 1, EFEMP1 ELISA Kit | MyBioSource MBS915805 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human EGF-containing fibulin-like extracellular matrix protein 1, EFEMP1 ELISA Kit

catalog :

MBS915805

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS915805

products type :

ELISA Kit

products full name :

Human EGF-containing fibulin-like extracellular matrix protein 1, EFEMP1 ELISA Kit

products short name :

EGF-containing fibulin-like extracellular matrix protein 1

products name syn :

Human EGF-containing fibulin-like extracellular matrix protein 1 (EFEMP1) ELISA kit; DHRD; DRAD; FBLN3; FBNL; FLJ35535; MGC111353; MLVT; MTLV; S1-5; fibrillin-like; fibulin 3; EGF-containing fibulin-like extracellular matrix protein 1

other names :

EGF-containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; EGF-containing fibulin-like extracellular matrix protein 1; fibulin-3; extracellular protein S1-5; EGF containing fibulin-like extracellular matrix protein 1; Extracellular protein S1-5; Fibrillin-like protein; Fibulin-3; FIBL-3

products gene name :

EFEMP1

other gene names :

EFEMP1; EFEMP1; DHRD; DRAD; FBNL; MLVT; MTLV; S1-5; FBLN3; FIBL-3; FBLN3; FBNL; FIBL-3

uniprot entry name :

FBLN3_HUMAN

reactivity :

Human

sequence length :

493

specificity :

This assay has high sensitivity and excellent specificity for detection of human EFEMP1. No significant cross-reactivity or interference between human EFEMP1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 1.56 ng/ml -100 ng/ml. Sensitivity: The minimum detectable dose of human EFEMP1 is typically less than 0.39 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EFEMP1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EFEMP1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EFEMP1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EFEMP1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

86788015

ncbi acc num :

NP_001034437.1

ncbi gb acc num :

NM_001039348.2

uniprot acc num :

Q12805

ncbi mol weight :

54,641 Da

ncbi summary :

This gene encodes a member of the fibulin family of extracellular matrix glycoproteins. Like all members of this family, the encoded protein contains tandemly repeated epidermal growth factor-like repeats followed by a C-terminus fibulin-type domain. This gene is upregulated in malignant gliomas and may play a role in the aggressive nature of these tumors. Mutations in this gene are associated with Doyne honeycomb retinal dystrophy. Alternatively spliced transcript variants that encode the same protein have been described.[provided by RefSeq, Nov 2009]

uniprot summary :

EFEMP1: Binds EGFR, the EGF receptor, inducing EGFR autophosphorylation and the activation of downstream signaling pathways. May play a role in cell adhesion and migration. May function as a negative regulator of chondrocyte differentiation. In the olfactory epithelium, it may regulate glial cell migration, differentiation and the ability of glial cells to support neuronal neurite outgrowth. Defects in EFEMP1 are a cause of Doyne honeycomb retinal dystrophy (DHRD); also known as malattia leventinese (MLVT) (ML). DHRD is an autosomal dominant disease characterized by yellow-white deposits known as drusen that accumulate beneath the retinal pigment epithelium. Belongs to the fibulin family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 2p16. Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; extracellular region. Molecular Function: protein binding; growth factor activity; epidermal growth factor receptor activity; calcium ion binding; epidermal growth factor receptor binding. Biological Process: epidermal growth factor receptor signaling pathway; extracellular matrix organization and biogenesis; peptidyl-tyrosine phosphorylation; visual perception; regulation of transcription, DNA-dependent; negative regulation of chondrocyte differentiation. Disease: Doyne Honeycomb Retinal Dystrophy

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage