Human Serine/threonine-protein kinase Sgk1, SGK1 ELISA Kit | MyBioSource MBS915643 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Serine/threonine-protein kinase Sgk1, SGK1 ELISA Kit

catalog :

MBS915643

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS915643

products type :

ELISA Kit

products full name :

Human Serine/threonine-protein kinase Sgk1, SGK1 ELISA Kit

products short name :

serum/glucocorticoid regulated kinase 1

products name syn :

Human Serine/threonine-protein kinase Sgk1 (SGK1) ELISA kit; RP1-188K17.1; SGK; serine/threonine protein kinase SGK; serum/glucocorticoid regulated kinase 1

other names :

serine/threonine-protein kinase Sgk1 isoform 2; Serine/threonine-protein kinase Sgk1; serine/threonine-protein kinase Sgk1; Sgk1 variant i3; serine/threonine protein kinase SGK; serum/glucocorticoid-regulated kinase 1; serum/glucocorticoid regulated kinase 1; Serum/glucocorticoid-regulated kinase 1

products gene name :

SGK1

other gene names :

SGK1; SGK1; SGK; SGK

uniprot entry name :

SGK1_HUMAN

reactivity :

Human

sequence length :

526

specificity :

This assay has high sensitivity and excellent specificity for detection of human SGK1. No significant cross-reactivity or interference between human SGK1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml -1500 pg/ml. Sensitivity: 5.86 pg/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SGK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SGK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SGK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SGK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

219521876

ncbi acc num :

NP_001137148.1

ncbi gb acc num :

NM_001143676.1

uniprot acc num :

O00141

ncbi mol weight :

48,942 Da

ncbi pathways :

Aldosterone-regulated Sodium Reabsorption Pathway (130626); Aldosterone-regulated Sodium Reabsorption Pathway (130590); Class I PI3K Signaling Events Pathway (138022); FoxO Family Signaling Pathway (138036); Glucocorticoid Receptor Regulatory Network Pathway (138014); IL-6 Signaling Pathway (198864); Insulin Pathway (138026); Insulin Signaling Pathway (198845); MTOR Signaling Pathway (138001)

ncbi summary :

This gene encodes a serine/threonine protein kinase that plays an important role in cellular stress response. This kinase activates certain potassium, sodium, and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability, and renal sodium excretion. High levels of expression of this gene may contribute to conditions such as hypertension and diabetic nephropathy. Several alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Jan 2009]

uniprot summary :

SGK1: serum and glucocorticoid-inducible kinase is an AGC protein kinase of the SGK family. Rapidly induced in response to a variety of stimuli including serum, glucocorticoids, follicle stimulating hormone, osmotic shock and mineralocorticoids. Can be activated via PI3K-dependent and -independent pathways. Plays a role in activating certain potassium, sodium and chloride channels, suggesting an involvement in the regulation of processes such as cell survival, neuronal excitability and renal sodium excretion. SGK is negatively regulated by ubiquitin modification and proteasome degradation. Protein type: Motility/polarity/chemotaxis; Kinase, protein; EC 2.7.11.1; Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); AGC group; SGK family. Chromosomal Location of Human Ortholog: 6q23. Cellular Component: nucleoplasm; endoplasmic reticulum membrane; mitochondrion; cytoplasm; plasma membrane; cytosol. Molecular Function: protein serine/threonine kinase activity; protein binding; potassium channel regulator activity; chloride channel regulator activity; sodium channel regulator activity; calcium channel regulator activity; protein serine/threonine/tyrosine kinase activity; ATP binding. Biological Process: apoptosis; positive regulation of transporter activity; sodium ion transport; protein amino acid phosphorylation; regulation of cell migration; regulation of cell proliferation; regulation of apoptosis; regulation of transcription factor activity; long-term memory; regulation of catalytic activity; regulation of blood pressure; response to stress; regulation of cell growth; transmembrane transport; response to DNA damage stimulus

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage