catalog number :
MBS915576
products type :
ELISA Kit
products full name :
Human Activity-regulated cytoskeleton-associated protein, ARC ELISA Kit
products short name :
activity-regulated cytoskeleton-associated protein
products name syn :
Human Activity-regulated cytoskeleton-associated protein (ARC) ELISA kit; Arg3.1; KIAA0278; ; activity-regulated cytoskeleton-associated protein
other names :
activity-regulated cytoskeleton-associated protein; Activity-regulated cytoskeleton-associated protein; activity-regulated cytoskeleton-associated protein; ARC/ARG3.1; activity-regulated gene 3.1 protein homolog; activity-regulated cytoskeleton-associated protein; ARC/ARG3.1; Activity-regulated gene 3.1 protein homolog; Arg3.1
other gene names :
ARC; ARC; Arg3.1; KIAA0278; Arg3.1
uniprot entry name :
ARC_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human ARC. No significant cross-reactivity or interference between human ARC and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: 6.25 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ARC has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARC present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARC is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARC bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_056008.1
ncbi gb acc num :
NM_015193.4
ncbi mol weight :
45,316 Da
ncbi pathways :
Amphetamine Addiction Pathway (547607); Amphetamine Addiction Pathway (550546)
uniprot summary :
ARG3.1: Required for consolidation of synaptic plasticity as well as formation of long-term memory. Regulates endocytosis of AMPA receptors in response to synaptic activity. Required for homeostatic synaptic scaling of AMPA receptors. Plays a role in the regulation of cell morphology and cytoskeletal organization. Required in the stress fiber dynamics and cell migration. Belongs to the ARC/ARG3.1 family. Chromosomal Location of Human Ortholog: 8q24.3. Cellular Component: postsynaptic membrane; cytoplasm; postsynaptic density; plasma membrane; dendritic spine; acrosome; cell junction; endosome; actin cytoskeleton. Biological Process: anterior/posterior pattern formation; cell migration; regulation of cell morphogenesis; cytoskeleton organization and biogenesis; endocytosis; learning; endoderm development; regulation of neuronal synaptic plasticity
size4 :
10x96-Strip-Wells