Human Protein S100-A4, S100A4 ELISA Kit | MyBioSource MBS915435 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Protein S100-A4, S100A4 ELISA Kit

catalog :

MBS915435

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS915435

products type :

ELISA Kit

products full name :

Human Protein S100-A4, S100A4 ELISA Kit

products short name :

S100 calcium binding protein A4

products name syn :

Human Protein S100-A4 (S100A4) ELISA kit; 18A2; 42A; CAPL; FSP1; MTS1; P9KA; PEL98; OTTHUMP00000015469; OTTHUMP00000032895; S100 calcium binding protein A4 (calcium protein; calvasculin; metastasin; murine placental homolog) ; S100 calcium-bind; S100 calcium binding protein A4

other names :

protein S100-A4; Protein S100-A4; protein S100-A4; protein Mts1; fibroblast-specific protein-1; placental calcium-binding protein; malignant transformation suppression 1; leukemia multidrug resistance associated protein; S100 calcium-binding protein A4 (calcium protein, calvasculin, metastasin, murine placental homolog); S100 calcium binding protein A4; Calvasculin; Metastasin; Placental calcium-binding protein; Protein Mts1; S100 calcium-binding protein A4

products gene name :

S100A4

other gene names :

S100A4; S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98; CAPL; MTS1

uniprot entry name :

S10A4_HUMAN

reactivity :

Human

sequence length :

101

specificity :

This assay has high sensitivity and excellent specificity for detection of human S100A4. No significant cross-reactivity or interference between human S100A4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, cell culture supernates, tissue homogenates. Detection Range: 0.9 ng/ml -60 ng/ml. Sensitivity: The minimum detectable dose of human S100A4 is typically less than 0.225 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Assay Type: Sandwich

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4506765

ncbi acc num :

NP_002952.1

ncbi gb acc num :

NM_002961.2

uniprot acc num :

P26447

ncbi mol weight :

11,729 Da

ncbi summary :

The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]

uniprot summary :

S100A4: is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in motility, invasion, and tubulin polymerization. Chromosomal rearrangements and altered expression of this gene have been implicated in tumor metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]. Protein type: Motility/polarity/chemotaxis. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extracellular space; neuron projection; perinuclear region of cytoplasm; nucleus. Molecular Function: identical protein binding; protein binding; RAGE receptor binding; calcium ion binding; actin binding; calcium-dependent protein binding. Biological Process: positive regulation of I-kappaB kinase/NF-kappaB cascade; epithelial to mesenchymal transition

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage