ELISA/assay

Human Ly6/PLAUR domain-containing protein 3, LYPD3 ELISA Kit

MBS915390

48-Strip-Wells

510 USD

ELISA Kit

Human Ly6/PLAUR domain-containing protein 3, LYPD3 ELISA Kit

LY6/PLAUR domain containing 3

Human Ly6/PLAUR domain-containing protein 3 (LYPD3) ELISA kit; C4.4A; 2310061G07Rik; GPI-anchored metastasis-associated protein homolog; LY6/PLAUR domain containing 3

ly6/PLAUR domain-containing protein 3; Ly6/PLAUR domain-containing protein 3; ly6/PLAUR domain-containing protein 3; MIG-C4; 2310061G07Rik; matrigel-induced gene C4 protein; GPI-anchored metastasis-associated protein homolog; GPI-anchored metastasis-associated protein C4.4A homolog; LY6/PLAUR domain containing 3; GPI-anchored metastasis-associated protein C4.4A homolog; Matrigel-induced gene C4 protein; MIG-C4

LYPD3

LYPD3; LYPD3; C4.4A; C4.4A; UNQ491/PRO1007; MIG-C4

LYPD3_HUMAN

Human

346

This assay has high sensitivity and excellent specificity for detection of human LYPD3. No significant cross-reactivity or interference between human LYPD3 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human LYPD3 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LYPD3 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LYPD3 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LYPD3 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LYPD3 bound in the initial step. The color development is stopped and the intensity of the color is measured.

93004088

NP_055215.2

NM_014400.2

O95274

35,971 Da

LYPD3: Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression. Protein type: Motility/polarity/chemotaxis; Cell adhesion; Membrane protein, GPI anchor. Chromosomal Location of Human Ortholog: 19q13.31. Cellular Component: extracellular space; anchored to plasma membrane; integral to membrane. Molecular Function: laminin binding. Biological Process: cell-matrix adhesion

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800