product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Nodal homolog, NODAL ELISA Kit
catalog :
MBS915258
quantity :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS915258
products type :
ELISA Kit
products full name :
Human Nodal homolog, NODAL ELISA Kit
products short name :
nodal homolog (mouse)
products name syn :
Human Nodal homolog (NODAL) ELISA kit; MGC138230; nodal; nodal homolog (mouse)
other names :
nodal homolog; Nodal homolog; nodal homolog; nodal growth differentiation factor
products gene name :
NODAL
other gene names :
NODAL; NODAL; HTX5
uniprot entry name :
NODAL_HUMAN
reactivity :
Human
sequence length :
347
specificity :
This assay has high sensitivity and excellent specificity for detection of human NODAL. No significant cross-reactivity or interference between human NODAL and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: For the quantitative determination of human nodal homolog (NODAL) concentrations in serum, plasma, tissue homogenates. Detection Range: 78 pg/ml -5000 pg/ml.
other info2 :
Sensitivity: The minimum detectable dose of human NODAL is typically less than 19.5 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NODAL has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NODAL present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NODAL is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NODAL bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
222352098
ncbi acc num :
NP_060525.3
ncbi gb acc num :
NM_018055.4
uniprot acc num :
Q96S42
ncbi mol weight :
39,561 Da
ncbi pathways :
Developmental Biology Pathway 477129!!Regulation Of Signaling By NODAL Pathway 477131!!Signaling By NODAL Pathway 477130!!TGF-beta Signaling Pathway 83064!!TGF-beta Signaling Pathway 475
ncbi summary :
The protein encoded by this gene is a member of the TGF-beta superfamily. Studies of the mouse counterpart suggested that this gene may be essential for mesoderm formation and subsequent organization of axial structures in early embryonic development. [provided by RefSeq, Jul 2008]
uniprot summary :
Function: Essential for mesoderm formation and axial patterning during embryonic development
size :
96 Strip Wells
price :
760 USD
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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