catalog number :
MBS915230
products type :
ELISA Kit
products full name :
Human Protein S100-A10, S100A10 ELISA Kit
products short name :
S100 calcium binding protein A10
products name syn :
Human Protein S100-A10 (S100A10) ELISA kit; 42C; ANX2L; ANX2LG; CAL1L; CLP11; Ca[1]; GP11; MGC111133; P11; p10; OTTHUMP00000015270; S100 calcium binding protein A10 (annexin II ligand; calpactin I; light polypeptide (p11) ) ; S100 calcium-binding; S100 calcium binding protein A10
other names :
protein S100-A10; Protein S100-A10; protein S100-A10; calpactin I light chain; calpactin-1 light chain; cellular ligand of annexin II; annexin II tetramer (AIIt) p11 subunit; annexin II ligand, calpactin I, light polypeptide; S100 calcium binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)); S100 calcium-binding protein A10 (annexin II ligand, calpactin I, light polypeptide (p11)); S100 calcium binding protein A10; Calpactin I light chain; Calpactin-1 light chain; Cellular ligand of annexin II; S100 calcium-binding protein A10; p10 protein; p11
products gene name :
S100A10
other gene names :
S100A10; S100A10; 42C; P11; p10; GP11; ANX2L; CAL1L; CLP11; Ca[1]; ANX2LG; ANX2LG; CAL1L; CLP11
uniprot entry name :
S10AA_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human S100A10. No significant cross-reactivity or interference between Human S100A10 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for S100A10 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any S100A10 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for S100A10 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of S100A10 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_002957.1
ncbi gb acc num :
NM_002966.2
ncbi mol weight :
11,203 Da
ncbi pathways :
Prostaglandin Synthesis And Regulation Pathway (198912)
ncbi summary :
The protein encoded by this gene is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and/or nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100 genes include at least 13 members which are located as a cluster on chromosome 1q21. This protein may function in exocytosis and endocytosis. [provided by RefSeq, Jul 2008]
uniprot summary :
S100A10: Because S100A10 induces the dimerization of ANXA2/p36, it may function as a regulator of protein phosphorylation in that the ANXA2 monomer is the preferred target (in vitro) of tyrosine- specific kinase. Heterotetramer containing 2 light chains of S100A10/p11 and 2 heavy chains of ANXA2/p36. Interacts with SCN10A. Belongs to the S-100 family. Protein type: Calcium-binding. Chromosomal Location of Human Ortholog: 1q21. Cellular Component: extrinsic to plasma membrane; lipid raft. Molecular Function: protein binding; protein homodimerization activity; calcium ion binding; lipid binding. Biological Process: positive regulation of focal adhesion formation; positive regulation of stress fiber formation; protein heterotetramerization; positive regulation of binding; lipid raft formation; membrane budding
size4 :
10x96-Strip-Wells