Human Platelet glycoprotein VI, GP6 ELISA Kit | MyBioSource MBS915183 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Platelet glycoprotein VI, GP6 ELISA Kit

catalog :

MBS915183

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS915183

products type :

ELISA Kit

products full name :

Human Platelet glycoprotein VI, GP6 ELISA Kit

products short name :

glycoprotein VI (platelet)

products name syn :

Human Platelet glycoprotein VI (GP6) ELISA kit; GPIV; GPVI; MGC138168; platelet collagen receptor; platelet glycoprotein VI; glycoprotein VI (platelet)

other names :

platelet glycoprotein VI isoform 1; Platelet glycoprotein VI; platelet glycoprotein VI; glycoprotein 6; platelet collagen receptor; glycoprotein VI (platelet); Glycoprotein 6

products gene name :

GP6

other gene names :

GP6; GP6; GPIV; GPVI; BDPLT11; GPVI

uniprot entry name :

GPVI_HUMAN

reactivity :

Human

sequence length :

620

specificity :

This assay has high sensitivity and excellent specificity for detection of human GP6. No significant cross-reactivity or interference between human GP6 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 46.88 pg/ml -3000 pg/ml. Sensitivity: The minimum detectable dose of human GP6 is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for GP6 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any GP6 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for GP6 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of GP6 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

563580292

ncbi acc num :

NP_001077368.2

ncbi gb acc num :

NM_001083899.2

uniprot acc num :

Q9HCN6

ncbi mol weight :

36,866 Da

ncbi pathways :

Cell Surface Interactions At The Vascular Wall Pathway (106062); ECM-receptor Interaction Pathway (83068); ECM-receptor Interaction Pathway (479); GPVI-mediated Activation Cascade Pathway (106036); Hemostasis Pathway (106028); Platelet Adhesion To Exposed Collagen Pathway (106030); Platelet Activation, Signaling And Aggregation Pathway (106034)

ncbi summary :

This gene encodes a platelet membrane glycoprotein of the immunoglobulin superfamily. The encoded protein is a receptor for collagen and plays a critical role in collagen-induced platelet aggregation and thrombus formation. The encoded protein forms a complex with the Fc receptor gamma-chain that initiates the platelet activation signaling cascade upon collagen binding. Mutations in this gene are a cause of platelet-type bleeding disorder-11 (BDPLT11). Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

uniprot summary :

GPVI: Collagen receptor involved in collagen-induced platelet adhesion and activation. Plays a key role in platelet procoagulant activity and subsequent thrombin and fibrin formation. This procoagulant function may contribute to arterial and venous thrombus formation. The signaling pathway involves the FcR gamma- chain, the Src kinases (likely Fyn/Lyn), the adapter protein LAT and leads to the activation of phospholipase C gamma2. Defects in GP6 are the cause of bleeding disorder platelet-type 11 (BDPLT11). BDPLT11 is a mild to moderate bleeding disorder caused by defective platelet activation and aggregation in response to collagen. 3 isoforms of the human protein are produced by alternative splicing. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 19q13.4. Cellular Component: cell surface; integral to plasma membrane; plasma membrane. Molecular Function: collagen binding; protein binding; transmembrane receptor activity; receptor activity. Biological Process: platelet activation; blood coagulation; leukocyte migration; enzyme linked receptor protein signaling pathway. Disease: Bleeding Disorder, Platelet-type, 11

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage