ELISA/assay

Rat Protein CYR61, CYR61 ELISA Kit

MBS915153

48-Strip-Wells

510 USD

ELISA Kit

Rat Protein CYR61, CYR61 ELISA Kit

cysteine-rich, angiogenic inducer, 61

Rat Protein CYR61 (CYR61) ELISA kit; CCN1; GIG1; IGFBP10; cysteine-rich heparin-binding protein 61; cysteine-rich; anigogenic inducer; 61; cysteine-rich; angiogenic inducer; 61

protein CYR61; Protein CYR61; protein CYR61; IBP-10; IGFBP-10; CCN family member 1; IGF-binding protein 10; cysteine rich protein 61; insulin-like growth factor-binding protein 10; cysteine-rich, angiogenic inducer, 61; CCN family member 1; Cysteine-rich angiogenic inducer 61; Insulin-like growth factor-binding protein 10; IBP-10; IGF-binding protein 10; IGFBP-10

CYR61

Cyr61; Cyr61; Ccn1; Igfbp10; IBP-10; IGF-binding protein 10; IGFBP-10

CYR61_RAT

Rat

379

This assay has high sensitivity and excellent specificity for detection of rat CYR61. No significant cross-reactivity or interference between rat CYR61 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of rat CYR61 is typically less than 7.81 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYR61 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYR61 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYR61 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYR61 bound in the initial step. The color development is stopped and the intensity of the color is measured.

31542447

NP_112617.2

NM_031327.2

Q9ES72

41,687 Da

Hypertrophy Model Pathway (198527)

an extracellular heparin binding protein involved in supporting smooth muscle cell adhesion, promoting cell migration, and enhancing growth factor-stimulated mitogenesis [RGD, Feb 2006]

Function: Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 CYR61-mediated gene regulation is dependent on heparin-binding Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800