ELISA/assay

Human Beta-2 adrenergic receptor, ADRB2 ELISA Kit

MBS915081

48-Strip-Wells

510 USD

ELISA Kit

Human Beta-2 adrenergic receptor, ADRB2 ELISA Kit

adrenergic, beta-2-, receptor, surface

Human Beta-2 adrenergic receptor (ADRB2) ELISA kit; ADRB2R; ADRBR; B2AR; BAR; BETA2AR; beta-2 adrenergic receptor; beta-2 adrenoceptor; catecholamine receptor; adrenergic; beta-2-; receptor; surface

beta-2 adrenergic receptor; Beta-2 adrenergic receptor; beta-2 adrenergic receptor; beta-2 adrenoceptor; beta-2 adrenoreceptor; catecholamine receptor; adrenoceptor beta 2 surface; adrenergic, beta-2-, receptor, surface; adrenoceptor beta 2, surface; Beta-2 adrenoreceptor; Beta-2 adrenoceptor

ADRB2

ADRB2; ADRB2; BAR; B2AR; ADRBR; ADRB2R; BETA2AR; ADRB2R; B2AR; Beta-2 adrenoceptor

ADRB2_HUMAN

Human

413

This assay has high sensitivity and excellent specificity for detection of human ADRB2. No significant cross-reactivity or interference between human ADRB2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 18.75 pg/ml -1200 pg/ml. Sensitivity: The minimum detectable dose of human ADRB2 is typically less than 4.69 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ADRB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ADRB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ADRB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ADRB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4501969

NP_000015.1

NM_000024.5

P07550

46,459 Da

Adrenoceptors Pathway (106365); Amine Ligand-binding Receptors Pathway (106363); Arf6 Signaling Events Pathway (138034); Arf6 Trafficking Events Pathway (137954); Calcium Regulation In The Cardiac Cell Pathway (198906); Calcium Signaling Pathway (83050); Calcium Signaling Pathway (459); Class A/1 (Rhodopsin-like Receptors) Pathway (106357); Endocytosis Pathway (102279); Endocytosis Pathway (102181)

This gene encodes beta-2-adrenergic receptor which is a member of the G protein-coupled receptor superfamily. This receptor is directly associated with one of its ultimate effectors, the class C L-type calcium channel Ca(V)1.2. This receptor-channel complex also contains a G protein, an adenylyl cyclase, cAMP-dependent kinase, and the counterbalancing phosphatase, PP2A. The assembly of the signaling complex provides a mechanism that ensures specific and rapid signaling by this G protein-coupled receptor. This gene is intronless. Different polymorphic forms, point mutations, and/or downregulation of this gene are associated with nocturnal asthma, obesity and type 2 diabetes. [provided by RefSeq, Jul 2008]

ADRB2: Beta-adrenergic receptors mediate the catecholamine- induced activation of adenylate cyclase through the action of G proteins. The beta-2-adrenergic receptor binds epinephrine with an approximately 30-fold greater affinity than it does norepinephrine. Belongs to the G-protein coupled receptor 1 family. Adrenergic receptor subfamily. ADRB2 sub-subfamily. Protein type: Membrane protein, multi-pass; Receptor, GPCR; GPCR, family 1; Membrane protein, integral. Chromosomal Location of Human Ortholog: 5q31-q32. Cellular Component: lysosome; integral to plasma membrane; apical plasma membrane; plasma membrane; nucleus; receptor complex; endosome. Molecular Function: protein binding; potassium channel regulator activity; protein homodimerization activity; beta2-adrenergic receptor activity; norepinephrine binding; adenylate cyclase binding. Biological Process: arrestin mediated desensitization of G-protein coupled receptor protein signaling pathway; receptor-mediated endocytosis; diet induced thermogenesis; negative regulation of multicellular organism growth; transmembrane receptor protein tyrosine kinase activation (dimerization); regulation of vasodilation; adenylate cyclase activation; regulation of sodium ion transport; positive regulation of bone mineralization; G-protein signaling, adenylate cyclase activating pathway; G-protein signaling, coupled to cAMP nucleotide second messenger; cell surface receptor linked signal transduction; positive regulation of MAPKKK cascade; positive regulation of protein ubiquitination; negative regulation of smooth muscle contraction; heat generation; brown fat cell differentiation; endosome to lysosome transport; positive regulation of transcription from RNA polymerase II promoter; response to cold; bone resorption; norepinephrine-epinephrine vasodilation involved in regulation of systemic arterial blood pressure. Disease: Obesity; Asthma, Susceptibility To

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800