ELISA/assay

Human Flotillin-1, FLOT1 ELISA Kit

MBS915065

48-Strip-Wells

510 USD

ELISA Kit

Human Flotillin-1, FLOT1 ELISA Kit

flotillin 1

Human Flotillin-1 (FLOT1) ELISA kit; DADB-118P11.2; integral membrane component of caveolae; flotillin 1

flotillin-1; Flotillin-1; flotillin-1; integral membrane component of caveolae; flotillin 1

FLOT1

FLOT1; FLOT1

FLOT1_HUMAN

Human

427

This assay has high sensitivity and excellent specificity for detection of human FLOT1. No significant cross-reactivity or interference between human FLOT1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 12.5 pg/ml -800 pg/ml. Sensitivity: The minimum detectable dose of human FLOT1 is typically less than 3.12 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FLOT1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FLOT1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FLOT1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FLOT1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

5031699

NP_005794.1

NM_005803.2

O75955

47,355 Da

Insulin Signaling Pathway (198845); Insulin Signaling Pathway (83090); Insulin Signaling Pathway (501)

Caveolae are small domains on the inner cell membrane involved in vesicular trafficking and signal transduction. FLOT1 encodes a caveolae-associated, integral membrane protein. The function of flotillin 1 has not been determined. [provided by RefSeq, Jul 2008]

FLOT1: May act as a scaffolding protein within caveolar membranes, functionally participating in formation of caveolae or caveolae-like vesicles. Heterooligomeric complex of flotillin-1 and flotillin-2 and caveolin-1 and caveolin-2. Interacts with ECM29. Belongs to the band 7/mec-2 family. Flotillin subfamily. Protein type: Adaptor/scaffold. Chromosomal Location of Human Ortholog: 6p21.3. Cellular Component: signalosome; focal adhesion; membrane; lysosomal membrane; melanosome; integral to membrane; microtubule organizing center; caveola; flotillin complex; endosome; sarcolemma; lipid raft. Molecular Function: ionotropic glutamate receptor binding; protein binding; protease binding. Biological Process: extracellular matrix disassembly; axonogenesis; regulation of Rho protein signal transduction

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800