Mouse Apolipoprotein A-IV, APOA4 ELISA Kit | MyBioSource MBS915059 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Apolipoprotein A-IV, APOA4 ELISA Kit

catalog :

MBS915059

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS915059

products type :

ELISA Kit

products full name :

Mouse Apolipoprotein A-IV, APOA4 ELISA Kit

products short name :

apolipoprotein A-IV

products name syn :

Mouse Apolipoprotein A-IV (APOA4) ELISA kit; MGC142154; MGC142156; ; apolipoprotein A-IV

other names :

apolipoprotein A-IV; Apolipoprotein A-IV; apolipoprotein A-IV; apo-AIV; apoA-IV; apolipoprotein A4; apolipoprotein A-IV; Apolipoprotein A4

products gene name :

APOA4

other gene names :

Apoa4; Apoa4; Apoa-4; Apo-AIV; ApoA-IV

uniprot entry name :

APOA4_MOUSE

reactivity :

Mouse

sequence length :

395

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse APOA4. No significant cross-reactivity or interference between mouse APOA4 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 12.5 ng/ml -800 ng/ml. Sensitivity: The minimum detectable dose of mouse APOA4 is typically less than 3.12 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for APOA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any APOA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for APOA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of APOA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

110347473

ncbi acc num :

NP_031494.2

ncbi gb acc num :

NM_007468.2

uniprot acc num :

P06728

ncbi mol weight :

45,029 Da

ncbi pathways :

Chylomicron-mediated Lipid Transport Pathway (574759); Fat Digestion And Absorption Pathway (194386); Fat Digestion And Absorption Pathway (194324); Lipid Digestion, Mobilization, And Transport Pathway (574755); Lipoprotein Metabolism Pathway (574758); Metabolism Pathway (574739); Metabolism Of Lipids And Lipoproteins Pathway (574754); Statin Pathway (198363); Vitamin Digestion And Absorption Pathway (199557); Vitamin Digestion And Absorption Pathway (199538)

uniprot summary :

APOA4: May have a role in chylomicrons and VLDL secretion and catabolism. Required for efficient activation of lipoprotein lipase by ApoC-II; potent activator of LCAT. Apoa-IV is a major component of HDL and chylomicrons. Synthesized primarily in the intestine and secreted in plasma. Belongs to the apolipoprotein A1/A4/E family. Protein type: Cell adhesion; Lipid-binding; Secreted, signal peptide; Endoplasmic reticulum; Secreted. Cellular Component: extracellular space; chylomicron; cell surface; extracellular region. Molecular Function: antioxidant activity; protein homodimerization activity; copper ion binding; cholesterol transporter activity; cholesterol binding; phosphatidylcholine binding; lipid binding. Biological Process: multicellular organismal lipid catabolic process; cholesterol metabolic process; positive regulation of fatty acid biosynthetic process; removal of superoxide radicals; protein-lipid complex assembly; neurite regeneration; lipid homeostasis; cholesterol efflux; lipoprotein metabolic process; lipid transport; cholesterol biosynthetic process; phosphatidylcholine metabolic process; leukocyte adhesion; cholesterol homeostasis; reverse cholesterol transport; innate immune response in mucosa; phospholipid efflux; regulation of cholesterol absorption; transport; response to lipid hydroperoxide; hydrogen peroxide catabolic process; positive regulation of lipoprotein lipase activity; regulation of cholesterol transport

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage