ELISA/assay

Human Nerve growth factor, NGF ELISA Kit

MBS914982

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Nerve growth factor, NGF ELISA Kit

[nerve growth factor (beta polypeptide)]

[Human Nerve growth factor; NGF ELISA kit; Beta-NGF; HSAN5; MGC161426; MGC161428; NGFB; beta-nerve growth factor; nerve growth factor; beta polypeptide; nerve growth factor; beta subunit; nerve growth factor (beta polypeptide)]

[beta-nerve growth factor; Beta-nerve growth factor; beta-nerve growth factor; nerve growth factor, beta subunit; nerve growth factor (beta polypeptide)]

[NGF]

[NGF; NGF; NGFB; HSAN5; Beta-NGF; NGFB; Beta-NGF]

NGF_HUMAN

Human

241

This assay has high sensitivity and excellent specificity for detection of human NGF. No significant cross-reactivity or interference between human NGF and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, cell culture supernates, tissue homogenates, cell lysates. Assay Type: Sandwich (Quantitative). Detection Range: 78 pg/ml-5000 pg/ml. Sensitivity: 19.5 pg/ml.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NGF has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NGF present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NGF is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NGF bound in the initial step. The color development is stopped and the intensity of the color is measured.

70995319

NP_002497.2

NM_002506.2

P01138

26,959 Da

ARMS-mediated Activation Pathway (106466); Activation Of TRKA Receptors Pathway (106456); Apoptosis Pathway (83060); Apoptosis Pathway (470); Axonal Growth Stimulation Pathway (106453); Cell Death Signalling Via NRAGE, NRIF And NADE Pathway (106443); Ceramide Signalling Pathway (106451); Frs2-mediated Activation Pathway (106465); Id Signaling Pathway (198871); MAPK Signaling Pathway (198779)

This gene is a member of the NGF-beta family and encodes a secreted protein which homodimerizes and is incorporated into a larger complex. This protein has nerve growth stimulating activity and the complex is involved in the regulation of growth and the differentiation of sympathetic and certain sensory neurons. Mutations in this gene have been associated with hereditary sensory and autonomic neuropathy, type 5 (HSAN5), and dysregulation of this gene's expression is associated with allergic rhinitis. [provided by RefSeq, Jul 2008]

NGF: Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades through those receptor tyrosine kinase to regulate neuronal proliferation, differentiation and survival. Homodimer. Belongs to the NGF-beta family. Protein type: Secreted, signal peptide; Secreted. Chromosomal Location of Human Ortholog: 1p13.1. Cellular Component: extracellular space; Golgi lumen; cytoplasmic membrane-bound vesicle; extracellular region; endosome. Molecular Function: metalloendopeptidase inhibitor activity; protein binding; nerve growth factor receptor binding; growth factor activity; receptor signaling protein activity. Biological Process: circadian rhythm; response to nicotine; response to peptide hormone stimulus; nerve growth factor receptor signaling pathway; activation of MAPKK activity; positive regulation of apoptosis; adult locomotory behavior; regulation of neuron differentiation; positive regulation of axon extension; response to glucocorticoid stimulus; regulation of neurotransmitter secretion; response to lipopolysaccharide; regulation of caspase activity; sensory perception of pain; regulation of axonogenesis; negative regulation of cell cycle; nerve growth factor processing; response to radiation; cell-cell signaling; small GTPase mediated signal transduction; negative regulation of neuron apoptosis; response to electrical stimulus; inflammatory response; response to drug; positive regulation of neuron maturation; phosphoinositide-mediated signaling; positive regulation of protein amino acid autophosphorylation; memory; regulation of release of sequestered calcium ion into cytosol; positive regulation of nerve growth factor receptor signaling pathway; peripheral nervous system development; neuron apoptosis; induction of apoptosis via death domain receptors; response to mechanical stimulus; phospholipase C activation; response to ozone; Ras protein signal transduction; positive regulation of transcription factor activity; positive regulation of axonogenesis; neurite morphogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; negative regulation of apoptosis. Disease: Neuropathy, Hereditary Sensory And Autonomic, Type V

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

450

96-Strip-Wells

640

5x96-Strip-Wells

2615

10x96-Strip-Wells

4940