Human Phospholipase D1, PLD1 ELISA Kit | MyBioSource MBS914961 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Phospholipase D1, PLD1 ELISA Kit

catalog :

MBS914961

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS914961

products type :

ELISA Kit

products full name :

Human Phospholipase D1, PLD1 ELISA Kit

products short name :

phospholipase D1, phosphatidylcholine-specific

products name syn :

Human Phospholipase D1 (PLD1) ELISA kit; choline phosphatase 1; phospholipase D1; phospholipase D1; phophatidylcholine-specific; phospholipase D1; phosphatidylcholine-specific

other names :

phospholipase D1 isoform a; Phospholipase D1; phospholipase D1; choline phosphatase 1; phosphatidylcholine-hydrolyzing phospholipase D1; phospholipase D1, phosphatidylcholine-specific; Choline phosphatase 1; Phosphatidylcholine-hydrolyzing phospholipase D1

products gene name :

PLD1

other gene names :

PLD1; PLD1; PLD 1; hPLD1

uniprot entry name :

PLD1_HUMAN

reactivity :

Human

sequence length :

1074

specificity :

This assay has high sensitivity and excellent specificity for detection of human PLD1. No significant cross-reactivity or interference between human PLD1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human PLD1 is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PLD1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PLD1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PLD1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PLD1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

4505873

ncbi acc num :

NP_002653.1

ncbi gb acc num :

NM_002662.4

uniprot acc num :

Q13393

ncbi mol weight :

124,184 Da

ncbi pathways :

Alpha-synuclein Signaling Pathway (137913); Arf6 Downstream Pathway (138038); Arf6 Trafficking Events Pathway (137954); CDC42 Signaling Events Pathway (137994); EGFR1 Signaling Pathway (198782); Endocytosis Pathway (102279); Endocytosis Pathway (102181); ErbB1 Downstream Signaling Pathway (138057); Ether Lipid Metabolism Pathway (82990); Ether Lipid Metabolism Pathway (365)

ncbi summary :

This gene encodes a phosphatidylcholine-specific phospholipase which catalyzes the hydrolysis of phosphatidylcholine in order to yield phosphatidic acid and choline. The enzyme may play a role in signal transduction and subcellular trafficking. Alternative splicing results in multiple transcript variants with both catalytic and regulatory properties. [provided by RefSeq, Sep 2011]

uniprot summary :

PLD1: a phosphatidylcholine-hydrolyzing phospholipase. Activated by ADP-ribosylated Rho and protein kinase C. Implicated as a critical step in numerous cellular pathways including membrane trafficking and the regulation polymorphonuclear leukocyte degranulation and oxidant production. May be involved in the regulation of perinuclear intravesicular membrane traffic. Four splice-variant isoforms have been described. Protein type: Lipid Metabolism - glycerophospholipid; Phospholipase; Motility/polarity/chemotaxis; Lipid Metabolism - ether lipid; EC 3.1.4.4. Chromosomal Location of Human Ortholog: 3q26. Cellular Component: Golgi membrane; Golgi apparatus; endoplasmic reticulum membrane; membrane; late endosome membrane; endocytic vesicle; perinuclear region of cytoplasm; lysosomal membrane; apical plasma membrane; endosome. Molecular Function: protein binding; phospholipase D activity; phosphoinositide binding. Biological Process: defense response to Gram-positive bacterium; phosphatidylglycerol biosynthetic process; small GTPase mediated signal transduction; phospholipid metabolic process; glycerophospholipid biosynthetic process; Ras protein signal transduction; phosphatidic acid biosynthetic process; chemotaxis; lipid catabolic process; regulation of microvillus biogenesis

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage