ELISA/assay

Human Ephrin type-A receptor 4, EPHA4 ELISA Kit

MBS914925

48-Strip-Wells

510 USD

ELISA Kit

Human Ephrin type-A receptor 4, EPHA4 ELISA Kit

EPH receptor A4

Human Ephrin type-A receptor 4 (EPHA4) ELISA kit; HEK8; SEK; TYRO1; OTTHUMP00000164185; TYRO1 protein tyrosine kinase; ephrin receptor EphA4; ephrin type-A receptor 4; receptor protein-tyrosine kinase HEK8; tyrosine-protein kinase receptor SEK; EPH receptor A4

ephrin type-A receptor 4; Ephrin type-A receptor 4; ephrin type-A receptor 4; EK8; EPH-like kinase 8; TYRO1 protein tyrosine kinase; tyrosine-protein kinase TYRO1; tyrosine-protein kinase receptor SEK; receptor protein-tyrosine kinase HEK8; EPH receptor A4; EPH-like kinase 8; EK8; hEK8; Tyrosine-protein kinase TYRO1; Tyrosine-protein kinase receptor SEK

EPHA4

EPHA4; EPHA4; SEK; HEK8; TYRO1; HEK8; SEK; TYRO1; EK8; hEK8

EPHA4_HUMAN

Human

986

This assay has high sensitivity and excellent specificity for detection of human EPHA4. No significant cross-reactivity or interference between human EPHA4 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 25 pg/ml -1600 pg/ml. Sensitivity: The minimum detectable dose of human EPHA4is typically less than 6.25 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Priciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

4758280

NP_004429.1

NM_004438.3

P54764

109,860 Da

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); EPHA Forward Signaling Pathway (138041); EphrinA-EPHA Pathway (137942)

This gene belongs to the ephrin receptor subfamily of the protein-tyrosine kinase family. EPH and EPH-related receptors have been implicated in mediating developmental events, particularly in the nervous system. Receptors in the EPH subfamily typically have a single kinase domain and an extracellular region containing a Cys-rich domain and 2 fibronectin type III repeats. The ephrin receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. [provided by RefSeq, Jul 2008]

EphA4: a tyrosine kinase receptor of the Eph family. Receptor for members of the ephrin-A family. Binds to ephrin-A1, -A4 and -A5. Binds more poorly to ephrin-A2 and -A3. May play a role in hindbrain pattern formation. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. Protein type: Protein kinase, TK; Kinase, protein; Protein kinase, tyrosine (receptor); EC 2.7.10.1; Membrane protein, integral; TK group; Eph family. Chromosomal Location of Human Ortholog: 2q36.1. Cellular Component: Golgi apparatus; cell surface; endoplasmic reticulum; integral to plasma membrane; postsynaptic density; dendrite; dendritic spine; perikaryon; postsynaptic membrane; mitochondrial outer membrane; early endosome membrane; axon; cytoplasm; plasma membrane; nerve terminal; neuromuscular junction; cell junction; filopodium. Molecular Function: identical protein binding; protein binding; ephrin receptor binding; transmembrane-ephrin receptor activity; GPI-linked ephrin receptor activity; PH domain binding; ATP binding; protein kinase activity. Biological Process: axon guidance; positive regulation of JNK activity; peptidyl-tyrosine phosphorylation; negative regulation of axon regeneration; protein amino acid autophosphorylation; ephrin receptor signaling pathway; regulation of astrocyte differentiation; motor axon guidance; positive regulation of dendrite morphogenesis; cell adhesion; corticospinal tract morphogenesis; glial cell migration; regulation of axonogenesis; adult walking behavior

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800