ELISA/assay

Rat Chitinase-3-like protein 1, CHI3L1 ELISA Kit

MBS914441

48-Strip-Wells

510 USD

ELISA Kit

Rat Chitinase-3-like protein 1, CHI3L1 ELISA Kit

chitinase 3-like 1 (cartilage glycoprotein-39)

Rat Chitinase-3-like protein 1 (CHI3L1) ELISA kit; ASRT7; DKFZp686N19119; FLJ38139; GP39; HC-gp39; HCGP-3P; YKL40; YYL-40; chitinase 3-like 1; chitinase 3-like 1 (cartilage glycoprotein-39)

chitinase-3-like protein 1; Chitinase-3-like protein 1; chitinase-3-like protein 1; GP-39; CGP-39; cartilage glycoprotein 39; chitinase 3-like 1 protein; chitinase 3-like 1 (cartilage glycoprotein-39); Cartilage glycoprotein 39; CGP-39; GP-39

CHI3L1

Chi3l1; Chi3l1; Chil1; CGP-39; GP-39

CH3L1_RAT

Rat

370

This assay has high sensitivity and excellent specificity for detection of rat CHI3L1. No significant cross-reactivity or interference between rat CHI3L1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.39 ng/ml -25 ng/ml. Sensitivity: The minimum detectable dose of rat CHI3L1 is typically less than 0.09 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHI3L1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHI3L1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHI3L1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHI3L1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

61657901

NP_446012.1

NM_053560.1

Q9WTV1

42,392 Da

Amino Sugar And Nucleotide Sugar Metabolism Pathway (83371); Amino Sugar And Nucleotide Sugar Metabolism Pathway (350)

human homolog plays a role in vascular smooth muscle cell migration and adhesion [RGD, Feb 2006]

Function: Carbohydrate-binding lectin with a preference for chitin. Has no chitinase activity. May play a role in tissue remodeling and in the capacity of cells to respond to and cope with changes in their environment. Plays a role in T-helper cell type 2 (Th2) inflammatory response and IL-13-induced inflammation, regulating allergen sensitization, inflammatory cell apoptosis, dendritic cell accumulation and M2 macrophage differentiation. Facilitates invasion of pathogenic enteric bacteria into colonic mucosa and lymphoid organs. Mediates activation of AKT1 signaling pathway and subsequent IL8 production in colonic epithelial cells. Regulates antibacterial responses in lung by contributing to macrophage bacterial killing, controlling bacterial dissemination and augmenting host tolerance. Also regulates hyperoxia-induced injury, inflammation and epithelial apoptosis in lung

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

10x96-Strip-Wells

4800