catalog number :
MBS914362
products type :
ELISA Kit
products full name :
Human C-type lectin domain family 4 member D, CLEC4D ELISA Kit
products short name :
C-type lectin domain family 4, member D
products name syn :
Human C-type lectin domain family 4 member D (CLEC4D) ELISA kit; CLEC-6; CLEC6; CLECSF8; MCL; MGC40078; MPCL; C-type (calcium dependent; carbohydrate-recognition domain) lectin; superfamily member 8; C-type lectin receptor; C-type lectin-like receptor 6; macrophage; C-type lectin domain family 4; member D
other names :
C-type lectin domain family 4 member D; C-type lectin domain family 4 member D; C-type lectin domain family 4 member D; C-type lectin receptor; macrophage C-type lectin; C-type lectin-like receptor 6; C-type lectin superfamily member 8; C-type (calcium dependent, carbohydrate-recognition domain) lectin, superfamily member 8; C-type lectin domain family 4, member D; C-type lectin superfamily member 8; C-type lectin-like receptor 6; CLEC-6
products gene name :
CLEC4D
other gene names :
CLEC4D; CLEC4D; MCL; MPCL; CLEC6; CLEC-6; CLECSF8; CLECSF8; MCL; CLEC-6
uniprot entry name :
CLC4D_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human CLEC4D. No significant cross-reactivity or interference between human CLEC4D and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 0.156 ng/ml -10 ng/ml. Sensitivity: The minimum detectable dose of human CLEC4D is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
. Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CLEC4D has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLEC4D present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLEC4D is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLEC4D bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_525126.2
ncbi gb acc num :
NM_080387.4
ncbi mol weight :
24,704 Da
ncbi summary :
This gene encodes a member of the C-type lectin/C-type lectin-like domain (CTL/CTLD) superfamily. Members of this family share a common protein fold and have diverse functions, such as cell adhesion, cell-cell signalling, glycoprotein turnover, and roles in inflammation and immune response. This gene is closely linked to other CTL/CTLD superfamily members on chromosome 12p13 in the natural killer gene complex region. [provided by RefSeq, Jul 2008]
uniprot summary :
CLEC4D: Functions as an endocytic receptor. May be involved in antigen uptake at the site of infection, either for clearance of the antigen, or for processing and further presentation to T cells. Protein type: Membrane protein, integral. Chromosomal Location of Human Ortholog: 12p13.31. Cellular Component: integral to membrane. Molecular Function: carbohydrate binding. Biological Process: innate immune response
size4 :
10x96-Strip-Wells