Human Solute carrier family 40 member 1, SLC40A1 ELISA Kit | MyBioSource MBS914349 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Solute carrier family 40 member 1, SLC40A1 ELISA Kit

catalog :

MBS914349

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS914349

products type :

ELISA Kit

products full name :

Human Solute carrier family 40 member 1, SLC40A1 ELISA Kit

products short name :

solute carrier family 40 (iron-regulated transporter) , member 1

products name syn :

Human Solute carrier family 40 member 1 (SLC40A1) ELISA kit; FPN1; HFE4; IREG1; MST079; MSTP079; MTP1; SLC11A3; ferroportin 1; iron regulated gene 1; putative ferroportin 1 variant IIIB; solute carrier family 11 (proton-coupled divalent metal ion transporters); ; solute carrier family 40 (iron-regulated transporter); member 1

other names :

solute carrier family 40 member 1; Solute carrier family 40 member 1; solute carrier family 40 member 1; iron regulated gene 1; putative ferroportin 1 variant IIIA; putative ferroportin 1 variant IIIB; solute carrier family 11 (proton-coupled divalent metal ion transporters), member 3; solute carrier family 40 (iron-regulated transporter), member 1; Ferroportin-1; Iron-regulated transporter 1

products gene name :

SLC40A1

other gene names :

SLC40A1; SLC40A1; FPN1; HFE4; MTP1; IREG1; MST079; MSTP079; SLC11A3; FPN1; IREG1; SLC11A3; MSTP079

uniprot entry name :

S40A1_HUMAN

reactivity :

Human

sequence length :

571

specificity :

This assay has high sensitivity and excellent specificity for detection of human SLC40A1. No significant cross-reactivity or interference between human SLC40A1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml -1500 pg/ml. Sensitivity: The minimum detectable dose of human SLC40A1 is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SLC40A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SLC40A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SLC40A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SLC40A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

7657100

ncbi acc num :

NP_055400.1

ncbi gb acc num :

NM_014585.5

uniprot acc num :

Q9NP59

ncbi mol weight :

62,542 Da

ncbi pathways :

Iron Uptake And Transport Pathway (187191); Metal Ion SLC Transporters Pathway (161066); Mineral Absorption Pathway (212237); Mineral Absorption Pathway (212220); SLC-mediated Transmembrane Transport Pathway (119558); Transmembrane Transport Of Small Molecules Pathway (106572); Transport Of Glucose And Other Sugars, Bile Salts And Organic Acids, Metal Ions And Amine Compounds Pathway (119571)

ncbi summary :

The protein encoded by this gene is a cell membrane protein that may be involved in iron export from duodenal epithelial cells. Defects in this gene are a cause of hemochromatosis type 4 (HFE4). [provided by RefSeq, Jul 2008]

uniprot summary :

SLC40A1: May be involved in iron export from duodenal epithelial cell and also in transfer of iron between maternal and fetal circulation. Mediates iron efflux in the presence of a ferroxidase (hephaestin and/or ceruloplasmin). Defects in SLC40A1 are the cause of hemochromatosis type 4 (HFE4). HFE4 is an autosomal dominant iron-loading disorder characterized by early iron accumulation in reticuloendothelial cells and a marked increase in serum ferritin before elevation of the transferrin saturation. Belongs to the ferroportin (FP) (TC 2.A.100) family. SLC40A subfamily. Protein type: Membrane protein, multi-pass; Membrane protein, integral; Transporter, SLC family; Transporter. Chromosomal Location of Human Ortholog: 2q32. Cellular Component: multivesicular body; synaptic vesicle; integral to plasma membrane; basolateral plasma membrane; cytoplasm; plasma membrane; integral to membrane; intracellular. Molecular Function: iron ion transmembrane transporter activity; protein binding. Biological Process: anatomical structure morphogenesis; cellular iron ion homeostasis; positive regulation of transcription from RNA polymerase II promoter; lymphocyte homeostasis; transmembrane transport; negative regulation of apoptosis. Disease: Hemochromatosis, Type 4

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage