Mouse NAD (P) H dehydrogenase [quinone] 1, NQO1 ELISA Kit | MyBioSource MBS914296 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse NAD (P) H dehydrogenase [quinone] 1, NQO1 ELISA Kit

catalog :

MBS914296

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS914296

products type :

ELISA Kit

products full name :

Mouse NAD (P) H dehydrogenase [quinone] 1, NQO1 ELISA Kit

products short name :

NAD (P) H dehydrogenase, quinone 1

products name syn :

Mouse NAD (P) H dehydrogenase [quinone] 1 (NQO1) ELISA kit; DHQU; DIA4; DTD; NMOR1; NMORI; QR1; DT-diaphorase; NAD (P) H:Quinone acceptor oxidoreductase type 1; NAD (P) H:menadione oxidoreductase 1; NAD (P) H:quinone oxireductase; azoreductase; diaphorase (NADH/NADPH) ; NAD (P) H dehydrogenase; quinone 1

other names :

NAD(P)H dehydrogenase; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; NAD(P)H dehydrogenase [quinone] 1; azoreductase; DT-diaphorase; menadione reductase; quinone reductase 1; phylloquinone reductase; diaphorase 4 (NADH/NADPH); NAD(P)H dehydrogenase (quinone); NAD(P)H:quinone oxidoreductase 1; NAD(P)H menadione oxidoreductase 1, dioxin inducible; NAD(P)H dehydrogenase, quinone 1; Azoreductase; DT-diaphorase; DTD; Menadione reductase; NAD(P)H:quinone oxidoreductase 1; Phylloquinone reductase; Quinone reductase 1; QR1

products gene name :

NQO1

other gene names :

Nqo1; Nqo1; Dtd; Ox1; Qr1; Dia4; Nmo1; Ox-1; Nmo-1; Nmor1; AV001255; Dia4; Nmo1; Nmor1; DTD; QR1

uniprot entry name :

NQO1_MOUSE

reactivity :

Mouse

sequence length :

274

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse NQO1. No significant cross-reactivity or interference between mouse NQO1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of mouse NQO1 is typically less than 7.81 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NQO1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NQO1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NQO1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NQO1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

94400773

ncbi acc num :

NP_032732.3

ncbi gb acc num :

NM_008706.5

uniprot acc num :

Q64669

ncbi mol weight :

30,960 Da

ncbi pathways :

Keap1-Nrf2 Pathway (198378); Metabolism Pathway (574739); Metabolism Of Amino Acids And Derivatives Pathway (574861); Oxidative Stress Pathway (198384); Regulation Of Ornithine Decarboxylase (ODC) Pathway (574875)

uniprot summary :

NQO1: a FAD-binding flavoprotein enzyme that that prevents the one electron reduction of quinones that results in the production of radical species. Involved in detoxification pathways as well as in biosynthetic processes such as the vitamin K-dependent gamma-carboxylation of glutamate residues in prothrombin synthesis. Mutations have been associated with tardive dyskinesia (TD), an increased risk of hematotoxicity after exposure to benzene, and susceptibility to various forms of cancer. The expression of NQO1 is increased in liver, colon and breast tumors and non-small cell lung cancer (NSCLC) compared with the normal tissues. Moreover, expression levels are also elevated in developing tumors, suggesting a role for NQ01 in the prevention of tumor development. A homozygous common missense variant (NQO1(*)2, rs1800566(T)), that disables NQO1 strongly predicts poor survival among two independent series of women with breast cancer. Studies on NQO1 knockout mice suggest that the lack of NQO1 enzymatic activity changes intracellular redox states resulting in a reduction in apoptosis, which in turn leads to myeloid hyperplasia of bone marrow. Altered expression is associated with Alzheimer s disease (AD). Inhibited by dicoumarol. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Oxidoreductase; EC 1.6.5.2. Cellular Component: cell soma; cytoplasm; cytosol. Molecular Function: identical protein binding; NAD(P)H dehydrogenase (quinone) activity; superoxide dismutase activity; oxidoreductase activity. Biological Process: removal of superoxide radicals; positive regulation of neuron apoptosis; negative regulation of catalytic activity; superoxide metabolic process; response to oxidative stress

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage