product summary
Loading...
company name :
MyBioSource
product type :
ELISA/assay
product name :
Pig Heme oxygenase 1, HMOX1 ELISA Kit
catalog :
MBS914287
quantity :
48-Strip-Wells
price :
565 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS914287
products type :
ELISA Kit
products full name :
Pig Heme oxygenase 1, HMOX1 ELISA Kit
products short name :
heme oxygenase (decycling) 1
products name syn :
Pig Heme oxygenase 1 (HMOX1) ELISA kit; HO-1; HSP32; bK286B10; heme oxygenase (decyclizing) 1; heme oxygenase (decycling) 1
other names :
heme oxygenase 1; Heme oxygenase 1; heme oxygenase 1; HO-1; heat shock protein 32
products gene name :
HMOX1
other gene names :
HMOX1; HMOX1; HSP32; HO-1
uniprot entry name :
HMOX1_PIG
reactivity :
Pig
sequence length :
288
specificity :
This assay has high sensitivity and excellent specificity for detection of pig HMOX1. No significant cross-reactivity or interference between pig HMOX1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.78 ng/ml -50 ng/ml. Sensitivity: The minimum detectable dose of pig HMOX1 is typically less than 0.195 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMOX1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMOX1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMOX1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMOX1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
51592105
ncbi acc num :
NP_001004027.1
ncbi gb acc num :
NM_001004027.1
uniprot acc num :
P32394
ncbi mol weight :
33,074 Da
ncbi pathways :
Mineral Absorption Pathway (212235); Mineral Absorption Pathway (212220); Porphyrin And Chlorophyll Metabolism Pathway (84440); Porphyrin And Chlorophyll Metabolism Pathway (407)
uniprot summary :
Function: Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis. Catalytic activity: Protoheme + 3 AH2 + 3 O2 = biliverdin + Fe2+ + CO + 3 A + 3 H2O.
size1 :
48-Strip-Wells
price1 :
565 USD
size2 :
96-Strip-Wells
price2 :
810
size3 :
5x96-Strip-Wells
price3 :
2750
size4 :
10x96-Strip-Wells
price4 :
5150
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

"MyBioSource's number 1 vision is to be the world's number 1 quality reagents provider."

Our goal is to provide researchers, scientists and customers alike with a one-stop-shop for all of their reagents needs, whether it is monoclonal antibody, polyclonal antibody, recombinant protein, peptide, etc...

"MyBioSource offers the best products at unbeatable prices."

Please spend a few minutes to browse our online catalogs and see the wide range of products available. We ship our products through our shipping/distribution facility in San Diego, California, USA.

Would you like to receive email and e-newsletter from MyBioSource about new products, special offers and events? Please click here to join our Mailing List!