Human Cartilage acidic protein 1, CRTAC1 ELISA Kit | MyBioSource MBS914205 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Cartilage acidic protein 1, CRTAC1 ELISA Kit

catalog :

MBS914205

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS914205

products type :

ELISA Kit

products full name :

Human Cartilage acidic protein 1, CRTAC1 ELISA Kit

products short name :

cartilage acidic protein 1

products name syn :

Human Cartilage acidic protein 1 (CRTAC1) ELISA kit; RP11-459F3.1; ASPIC1; CEP-68; FLJ10320; acidic secreted protein in cartilage; chondrocyte expressed protein 68 kDa CEP-68; cartilage acidic protein 1

other names :

cartilage acidic protein 1 isoform B; Cartilage acidic protein 1; cartilage acidic protein 1; acidic secreted protein in cartilage; chondrocyte expressed protein 68 kDa CEP-68; cartilage acidic protein 1; 68 kDa chondrocyte-expressed protein; CEP-68; ASPIC

products gene name :

CRTAC1

other gene names :

CRTAC1; CRTAC1; ASPIC; ASPIC1; CEP-68; ASPIC1; CEP68; CEP-68

uniprot entry name :

CRAC1_HUMAN

reactivity :

Human

sequence length :

645

specificity :

This assay has high sensitivity and excellent specificity for detection of human CRTAC1. No significant cross-reactivity or interference between human CRTAC1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 4.68 ng/ml-300 ng/ml. Sensitivity: The minimum detectable dose of human CRTAC1 is typically less than 1.17 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CRTAC1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CRTAC1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CRTAC1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CRTAC1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

330688397

ncbi acc num :

NP_001193457.1

ncbi gb acc num :

NM_001206528.2

uniprot acc num :

Q9NQ79

ncbi mol weight :

71,421 Da

ncbi summary :

This gene encodes a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]

uniprot summary :

CRTAC1: a glycosylated extracellular matrix protein that is found in the interterritorial matrix of articular deep zone cartilage. This protein is used as a marker to distinguish chondrocytes from osteoblasts and mesenchymal stem cells in culture. The presence of FG-GAP motifs and an RGD integrin-binding motif suggests that this protein may be involved in cell-cell or cell-matrix interactions. Copy number alterations in this gene have been observed in neurofibromatosis type 1-associated glomus tumors. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2011]. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 10q22. Cellular Component: proteinaceous extracellular matrix; growth cone. Molecular Function: calcium ion binding. Biological Process: olfactory bulb development; axonal fasciculation

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage