Rabbit IL-6 ELISA Kit | MyBioSource MBS9141588 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Rabbit IL-6 ELISA Kit

catalog :

MBS9141588

quantity :

96 Tests

price :

480 USD

product information

catalog number :

MBS9141588

products type :

ELISA Kit

products full name :

Rabbit IL-6 ELISA Kit

products short name :

[IL-6]

products name syn :

[Rabbit Interleukin 6]

other names :

[interleukin-6; Interleukin-6; interleukin-6; interleukin 6]

products gene name :

[IL-6]

other gene names :

[Il6; Il6; ILg6; Ifnb2; Il-6; IL-6]

uniprot entry name :

IL6_RAT

reactivity :

Rabbit

sequence length :

211

storage stability :

Store at 4 degree C.

other info1 :

Assay Type: Competitive ELISA. Samples: Cell Culture Supernatant, Urine, Serum, Plasma. Detection Range: 50-1000 pg/mL

other info2 :

Conjugate: HRP. Detection Principle: Colorimetric

products description :

Intended Use: This Rabbit IL-6 ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of Rabbit IL-6 in the sample, this Rabbit IL-6 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of optical density versus Rabbit IL-6 concentration. The concentration of the samples is then determined by comparing the O.D. of the samples to the standard curve. Overview: The ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of samples in cell culture supernatant, serum, plasma (EDTA, citrate, heparin). Background: As an analytic biochemistry assay, ELISA involves detection of an 'analyte' (i.e. the specific substance whose presence is being quantitatively or qualitatively analyzed) in a liquid sample by a method that continues to use liquid reagents during the 'analysis' (i.e. controlled sequence of biochemical reactions that will generate a signal which can be easily quantified and interpreted as a measure of the amount of analyte in the sample) that stays liquid and remains inside a reaction chamber or well needed to keep the reactants contained.As a heterogenous assay, ELISA separates some component of the analytical reaction mixture by adsorbing certain components onto a solid phase which is physically immobilized. In ELISA, a liquid sample is added onto a stationary solid phase with special binding properties and is followed by multiple liquid reagents that are sequentially added, incubated and washed followed by some optical change (e.g. color development by the product of an enzymatic reaction) in the final liquid in the well from which the quantity of the analyte is measured. The qualitative 'reading' usually based on detection of intensity of transmitted light by spectrophotometry, which involves quantitation of transmission of some specific wavelength of light through the liquid (as well as the transparent bottom of the well in the multiple-well plate format). The sensitivity of detection depends on amplification of the signal during the analytic reactions. Since enzyme reactions are very well known amplification processes, the signal is generated by enzymes which are linked to the detection reagents in fixed proportions to allow accurate quantification-thus the name 'enzyme linked'.The analyte is also called the ligand because it will specifically bind or ligate to a detection reagent, thus ELISA falls under the bigger category of ligand binding assays. The ligand-specific binding reagent is 'immobilized', i.e., usually coated and dried onto the transparent bottom and sometimes also side wall of a well (the stationary 'solid phase 'solid substrate' here as opposed to solid microparticle/beads that can be washed away), which is usually constructed as a multiple-well plate known as the 'ELISA plate'. Conventionally, like other forms of immunoassays, the specificity of antigen-antibody type reaction is used because it is easy to raise an antibody specifically against an antigen in bulk as a reagent. Alternatively, if the analyte itself is an antibody, its target antigen can be used as the binding reagent.

ncbi gi num :

7549769

ncbi gb acc num :

NP_036721.1

uniprot acc num :

P20607

ncbi pathways :

Adipogenesis Pathway (198479); African Trypanosomiasis Pathway (194377); African Trypanosomiasis Pathway (194323); Amoebiasis Pathway (167311); Amoebiasis Pathway (167191); Androgen Receptor Signaling Pathway (198529); Chagas Disease (American Trypanosomiasis) Pathway (147806); Chagas Disease (American Trypanosomiasis) Pathway (147795); Cytokine Signaling In Immune System Pathway (829152); Cytokine-cytokine Receptor Interaction Pathway (83443)

ncbi summary :

a cytokine involved in development and possibly in neurodegenerative processes [RGD, Feb 2006]

uniprot summary :

Function: Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoietic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance . By similarity. Subcellular location: Secreted. Sequence similarities: Belongs to the IL-6 superfamily.

size1 :

96 Tests

price1 :

480 USD