Human Arachidonate 15-lipoxygenase B, ALOX15B ELISA Kit | MyBioSource MBS914061 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Arachidonate 15-lipoxygenase B, ALOX15B ELISA Kit

catalog :

MBS914061

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

citations: 1

Reference
Wenzel S, Tyurina Y, Zhao J, St Croix C, Dar H, Mao G, et al. PEBP1 Wardens Ferroptosis by Enabling Lipoxygenase Generation of Lipid Death Signals. Cell. 2017;171:628-641.e26 pubmed publisher

product information

catalog number :

MBS914061

products type :

ELISA Kit

products full name :

Human Arachidonate 15-lipoxygenase B, ALOX15B ELISA Kit

products short name :

arachidonate 15-lipoxygenase, type B

products name syn :

Human Arachidonate 15-lipoxygenase B (ALOX15B) ELISA kit; 15-LOX-2; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; arachidonate 15-lipoxygenase; second type; arachidonate omega (6) lipoxygenase; arachidonate 15-lipoxygenase; type B

other names :

arachidonate 15-lipoxygenase B isoform a; Arachidonate 15-lipoxygenase B; arachidonate 15-lipoxygenase B; 15-LOX-B; 15S-lipoxygenase; arachidonate 15-lipoxygenase 2; linoleate 13-lipoxygenase 15-LOb; arachidonate omega(6) lipoxygenase; arachidonate 15-lipoxygenase type II; arachidonate 15-lipoxygenase, second type; arachidonate 15-lipoxygenase, type B; 15-lipoxygenase 2; 15-LOX-2; Arachidonate 15-lipoxygenase type II; Linoleate 13-lipoxygenase 15-LOb (EC:1.13.11.-)

products gene name :

ALOX15B

other gene names :

ALOX15B; ALOX15B; 15-LOX-2; 15-LOX-B; 15-LOX-2

uniprot entry name :

LX15B_HUMAN

reactivity :

Human

sequence length :

647

specificity :

This assay has high sensitivity and excellent specificity for detection of human ALOX15B. No significant cross-reactivity or interference between human ALOX15B and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 28 pg/ml -1800 pg/ml. Sensitivity: 7 pg/ml.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for ALOX15B has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ALOX15B present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ALOX15B is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ALOX15B bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

85067499

ncbi acc num :

NP_001034219.1

ncbi gb acc num :

NM_001039130.1

uniprot acc num :

O15296

ncbi mol weight :

75,857 Da

ncbi pathways :

Arachidonic Acid Metabolism Pathway (82991); Arachidonic Acid Metabolism Pathway (366); Eicosanoid Synthesis Pathway (198888); Selenium Pathway (198825); Serotonergic Synapse Pathway (525336)

ncbi summary :

This gene encodes a member of the lipoxygenase family of structurally related nonheme iron dioxygenases involved in the production of fatty acid hydroperoxides. The encoded protein converts arachidonic acid exclusively to 15S-hydroperoxyeicosatetraenoic acid, while metabolizing linoleic acid less effectively. This gene is located in a cluster of related genes and a pseudogene that spans approximately 100 kilobases on the short arm of chromosome 17. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]

uniprot summary :

ALOX15B: Converts arachidonic acid exclusively to 15S- hydroperoxyeicosatetraenoic acid, while linoleic acid is less well metabolized. Belongs to the lipoxygenase family. 4 isoforms of the human protein are produced by alternative splicing. Protein type: Lipid Metabolism - arachidonic acid; Motility/polarity/chemotaxis; Oxidoreductase; EC 1.13.11.33; Cell cycle regulation; Apoptosis. Chromosomal Location of Human Ortholog: 17p13.1. Cellular Component: extrinsic to membrane; cytoskeleton; membrane; cytoplasm; plasma membrane; intracellular; cytosol; nucleus. Molecular Function: arachidonate 15-lipoxygenase activity; lipoxygenase activity; iron ion binding; calcium ion binding; lipid binding. Biological Process: prostate gland development; negative regulation of cell proliferation; lipoxygenase pathway; apoptosis; regulation of epithelial cell differentiation; linoleic acid metabolic process; arachidonic acid metabolic process; hepoxilin biosynthetic process; lipid metabolic process; negative regulation of cell migration; negative regulation of growth; positive regulation of keratinocyte differentiation; negative regulation of cell cycle

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage