catalog number :
MBS914018
products type :
ELISA Kit
products full name :
Mouse Neurturin, NRTN ELISA Kit
products short name :
neurturin
products name syn :
Mouse Neurturin (NRTN) ELISA kit; NTN; ; neurturin
other names :
neurturin; Neurturin; neurturin; neurturin
products gene name :
NRTN
other gene names :
Nrtn; Nrtn; NTN
uniprot entry name :
NRTN_MOUSE
specificity :
This assay has high sensitivity and excellent specificity for detection of mouse NRTN. No significant cross-reactivity or interference between mouse NRTN and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: 7.81 pg/ml.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for NRTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any NRTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for NRTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of NRTN bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_032764.1
ncbi gb acc num :
NM_008738.2
ncbi mol weight :
22,219 Da
ncbi pathways :
Axon Guidance Pathway (575211); Developmental Biology Pathway (575210); NCAM Signaling For Neurite Out-growth Pathway (575220); NCAM1 Interactions Pathway (575221)
uniprot summary :
NRTN: Supports the survival of sympathetic neurons in culture. May regulate the development and maintenance of the CNS. Might control the size of non-neuronal cell population such as haemopoietic cells. Genetic variations in NRTN may contribute to Hirschsprung disease, in association with mutations of RET gene, and possibly mutations in other loci. Hirschsprung disease is a disorder of neural crest development is characterized by the absence of intramural ganglion cells in the hindgut, often resulting in intestinal obstruction. Belongs to the TGF-beta family. GDNF subfamily. Protein type: Secreted, signal peptide; Secreted. Cellular Component: axon; extracellular region. Molecular Function: growth factor activity. Biological Process: nerve development; neural crest cell migration; neurite development; transmembrane receptor protein tyrosine kinase signaling pathway. Disease: Hirschsprung Disease, Susceptibility To, 1
size4 :
10x96-Strip-Wells