product summary
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company name :
MyBioSource
product type :
ELISA/assay
product name :
Human Beta-defensin 104, DEFB104A ELISA Kit
catalog :
MBS913953
quantity :
48-Strip-Wells
price :
510 USD
more info or order :
MyBioSource product webpage
product information
catalog number :
MBS913953
products type :
ELISA Kit
products full name :
Human Beta-defensin 104, DEFB104A ELISA Kit
products short name :
defensin, beta 104A
products name syn :
Human Beta-defensin 104 (DEFB104A) ELISA kit; BD-4; DEFB-4; DEFB104; DEFB4; MGC118942; MGC118944; MGC118945; hBD-4; defensin; beta 4; defensin; beta 104A
other names :
beta-defensin 104; Beta-defensin 104; beta-defensin 104; beta-defensin 4; defensin, beta 104; defensin, beta 104B; Beta-defensin 4; BD-4; DEFB-4; hBD-4; Defensin, beta 104
products gene name :
DEFB104A
other gene names :
DEFB104B; DEFB104A; BD-4; hBD-4; DEFB-4; DEFB104; DEFB4 DEFB104B ; BD-4; DEFB-4; hBD-4
uniprot entry name :
D104A_HUMAN
reactivity :
Human
sequence length :
72
specificity :
This assay has high sensitivity and excellent specificity for detection of human DEFB104A. No significant cross-reactivity or interference between human DEFB104A and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, cell culture supernates, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.25 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of human DEFB104A is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFB104A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFB104A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFB104A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFB104A bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi gi num :
103485137
ncbi acc num :
NP_001035792.1
ncbi gb acc num :
NM_001040702.1
uniprot acc num :
Q8WTQ1
ncbi mol weight :
8,526 Da
ncbi pathways :
Beta Defensins Pathway (530759); Defensins Pathway (530757); Immune System Pathway (106386); Innate Immune System Pathway (106387)
ncbi summary :
Defensins form a family of microbicidal and cytotoxic peptides made by neutrophils. Defensins are short, processed peptide molecules that are classified by structure into three groups: alpha-defensins, beta-defensins and theta-defensins. All beta-defensin genes are densely clustered in four to five syntenic chromosomal regions. Chromosome 8p23 contains at least two copies of the duplicated beta-defensin cluster. This duplication results in two identical copies of defensin, beta 104, DEFB104A and DEFB104B, in head-to-head orientation. This gene, DEFB104B, represents the more telomeric copy. [provided by RefSeq, Jul 2008]
uniprot summary :
defensin, beta 4: Has antimicrobial activity. Synergistic effects with lysozyme and DEFB103. Belongs to the beta-defensin family. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 8p23.1. Cellular Component: cell surface; extracellular region. Biological Process: defense response to bacterium; innate immune response
size1 :
48-Strip-Wells
price1 :
510 USD
size2 :
96-Strip-Wells
price2 :
725
size3 :
5x96-Strip-Wells
price3 :
2565
size4 :
10x96-Strip-Wells
price4 :
4800
more info or order :
MyBioSource product webpage
company information
MyBioSource
P.O. Box 153308
San Diego, CA 92195-3308
sales@mybiosource.com
https://www.mybiosource.com
1-888-627-0165
headquarters: USA
MyBioSource, LLC was orginally founded in Vancouver by three enthusiastic scientists who are passionate about providing the world with the best reagents available. Together, they form a company with a big vision known as MyBioSource. MyBioSource is now located in San Diego, California, USA.

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