Human Ephrin type-B receptor 2, EPHB2 ELISA Kit | MyBioSource MBS913951 product information

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product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Ephrin type-B receptor 2, EPHB2 ELISA Kit

catalog :

MBS913951

quantity :

24-Strip-Wells (LIMIT 1)

price :

240 USD

product information

catalog number :

MBS913951

products type :

ELISA Kit

products full name :

Human Ephrin type-B receptor 2, EPHB2 ELISA Kit

products short name :

[EPH receptor B2]

products name syn :

[Human Ephrin type-B receptor 2 (EPHB2) ELISA kit; CAPB; DRT; EPHT3; ERK; Hek5; MGC87492; PCBC; Tyro5; elk-related tyrosine kinase; eph tyrosine kinase 3; ephrin type-B receptor 2; protein-tyrosine kinase HEK5; EPH receptor B2]

other names :

[ephrin type-B receptor 2 isoform 2; Ephrin type-B receptor 2; ephrin type-B receptor 2; EPH-like kinase 5; eph tyrosine kinase 3; elk-related tyrosine kinase; protein-tyrosine kinase HEK5; tyrosine-protein kinase TYRO5; renal carcinoma antigen NY-REN-47; tyrosine-protein kinase receptor EPH-3; developmentally-regulated Eph-related tyrosine kinase; EPH receptor B2; Developmentally-regulated Eph-related tyrosine kinase; ELK-related tyrosine kinase; EPH tyrosine kinase 3; EPH-like kinase 5; EK5; hEK5; Renal carcinoma antigen NY-REN-47; Tyrosine-protein kinase TYRO5; Tyrosine-protein kinase receptor EPH-3]

products gene name :

[EPHB2]

other gene names :

[EPHB2; EPHB2; DRT; EK5; ERK; CAPB; Hek5; PCBC; EPHT3; Tyro5; DRT; EPHT3; EPTH3; ERK; HEK5; TYRO5; EK5; hEK5]

uniprot entry name :

EPHB2_HUMAN

reactivity :

Human

sequence length :

987

specificity :

This assay has high sensitivity and excellent specificity for detection of human EPHB2. No significant cross-reactivity or interference between human EPHB2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

image1 heading :

Typical Testing Data/Standard Curve (for reference only)

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Quantitative Sandwich. Detection Range: 0.625 ng/ml - 40 ng/ml. Sensitivity: 0.156 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for EPHB2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any EPHB2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for EPHB2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of EPHB2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

21396504

ncbi acc num :

NP_004433.2

ncbi gb acc num :

NM_004442.6

uniprot acc num :

P29323

ncbi mol weight :

117,493 Da

ncbi pathways :

Axon Guidance Pathway (83065); Axon Guidance Pathway (476); Axon Guidance Pathway (105688); Developmental Biology Pathway (477129); EPHB Forward Signaling Pathway (138047); Ephrin B Reverse Signaling Pathway (138052); EphrinB-EPHB Pathway (137951); Keap1-Nrf2 Pathway (198777); L1CAM Interactions Pathway (161003); NLR Proteins Pathway (198767)

ncbi summary :

Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene is a receptor for ephrin-B family members. [provided by RefSeq, Jul 2008]

uniprot summary :

EphB2: a receptor tyrosine kinase of the Eph family. A receptor for ephrin-B family members. Activated EphB2 recruits RasGAP, down-regulating the Ras-Erk signaling axis and neurite retraction. The Eph receptor tyrosine kinase family, the largest in the tyrosine kinase group, has fourteen members. They bind membrane-anchored ligands, ephrins, at sites of cell-cell contact, regulating the repulsion and adhesion of cells that underlie the establishment, maintenance, and remodeling of patterns of cellular organization. Eph signals are particularly important in regulating cell adhesion and cell migration during development, axon guidance, homeostasis and disease. EphA receptors bind to GPI-anchored ephrin-A ligands, while EphB receptors bind to ephrin-B proteins that have a transmembrane and cytoplasmic domain. Interactions between EphB receptor kinases and ephrin-B proteins transduce signals bidirectionally, signaling to both interacting cell types. Eph receptors and ephrins also regulate the adhesion of endothelial cells and are required for the remodeling of blood vessels. The ligand-activated form of EphB2 interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. Point mutations seen in prostate cancer. Overexpressed and required for migration of glioblastoma. Overexpressed and correlated with poor survival in breast cancer. Overexpression and loss of heterozygosity seen in colorectal cancers. Target for immunoconjugate drug therapy .Three splice-variant isoforms have been described. Protein type: Tumor suppressor; Membrane protein, integral; Protein kinase, TK; EC 2.7.10.1; Kinase, protein; Protein kinase, tyrosine (receptor); TK group; Eph family. Chromosomal Location of Human Ortholog: 1p36.1-p35. Cellular Component: cell soma; integral to plasma membrane; axon; dendrite; extracellular region; plasma membrane; cytosol. Molecular Function: protein binding; transmembrane-ephrin receptor activity; protein-tyrosine kinase activity; axon guidance receptor activity; ATP binding; receptor binding. Biological Process: nervous system development; axon guidance; inner ear morphogenesis; peptidyl-tyrosine phosphorylation; axonal fasciculation; central nervous system projection neuron axonogenesis; palate development; optic nerve morphogenesis; learning; positive regulation of long-term neuronal synaptic plasticity; corpus callosum development; positive regulation of synaptogenesis; regulation of body fluid levels; camera-type eye morphogenesis; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; phosphorylation; urogenital system development; retinal ganglion cell axon guidance. Disease: Prostate Cancer/brain Cancer Susceptibility

size1 :

24-Strip-Wells (LIMIT 1)

price1 :

240 USD

size2 :

48-Strip-Wells

price2 :

495

size3 :

96-Strip-Wells

price3 :

710

size4 :

5x96-Strip-Wells

price4 :

2705

size5 :

10x96-Strip-Wells

price5 :

5145