ELISA/assay

Rat Creatine Kinase MB Isoenzyme ELISA Kit (CKMB)

MBS9139097

96 Tests

510 USD

ELISA Kit

Rat Creatine Kinase MB Isoenzyme ELISA Kit (CKMB)

[Creatine Kinase MB Isoenzyme (CKMB)]

[CKMB]

Rat

This assay has high sensitivity and excellent specificity for detection of C3a.

Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Samples: Mouse Serum, Plasma, Tissue Homogenates, Cell Lysates, Cell Culture Supernates And Other Biological Fluids. Sensitivity: 6.1 pg/mL

Intra-assay Precision: 3 samples with low, middle and high level C3a were tested 20 times on one plate, respectively. Intra-Assay: CV<10%. Inter-assay Precision: 3 samples with low, middle and high level C3a were tested on 3 different plates, 8 replicates in each plate. Inter-Assay: CV<12%

Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to C3a. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to C3a. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain C3a, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm+/-10nm. The concentration of C3a in the samples is then determined by comparing the O.D. of the samples to the standard curve

96 Tests

510 USD