ELISA/assay

Human Titin, TTN ELISA Kit

MBS913885

24-Strip-Wells (LIMIT 1)

240 USD

ELISA Kit

Human Titin, TTN ELISA Kit

[titin]

[Human Titin (TTN) ELISA kit; CMD1G; CMH9; CMPD4; CONNECTIN; DKFZp451N061; EOMFC; FLJ26020; FLJ26409; FLJ32040; FLJ34413; FLJ39564; FLJ43066; HMERF; LGMD2J; TMD; rhabdomyosarcoma antigen MU-RMS-40.14; titin]

[titin isoform N2BA; Titin; titin; connectin; rhabdomyosarcoma antigen MU-RMS-40.14; titin; Connectin; Rhabdomyosarcoma antigen MU-RMS-40.14]

[TTN]

[TTN; TTN; TMD; CMH9; CMD1G; CMPD4; EOMFC; HMERF; MYLK5; LGMD2J]

TITIN_HUMAN

Human

34350

This assay has high sensitivity and excellent specificity for detection of Human TTN. No significant cross-reactivity or interference between Human TTN and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Samples: Serum, plasma, tissue homogenates, Cell lysates. Assay Type: Sandwich. Detection Range: 23.5 pg/ml-1500 pg/ml. Sensitivity: 5.8 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TTN has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TTN present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TTN is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TTN bound in the initial step. The color development is stopped and the intensity of the color is measured.

378925625

NP_001243779.1

NM_001256850.1

Q8WZ42

3,816,030 Da

Dilated Cardiomyopathy Pathway (121494); Dilated Cardiomyopathy Pathway (121285); Hemostasis Pathway (106028); Hypertrophic Cardiomyopathy (HCM) Pathway (114229); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (106261); Platelet Activation, Signaling And Aggregation Pathway (106034); Platelet Degranulation Pathway (106050); Response To Elevated Platelet Cytosolic Ca2+ Pathway (106048); Striated Muscle Contraction Pathway (106262)

This gene encodes a large abundant protein of striated muscle. The product of this gene is divided into two regions, a N-terminal I-band and a C-terminal A-band. The I-band, which is the elastic part of the molecule, contains two regions of tandem immunoglobulin domains on either side of a PEVK region that is rich in proline, glutamate, valine and lysine. The A-band, which is thought to act as a protein-ruler, contains a mixture of immunoglobulin and fibronectin repeats, and possesses kinase activity. An N-terminal Z-disc region and a C-terminal M-line region bind to the Z-line and M-line of the sarcomere, respectively, so that a single titin molecule spans half the length of a sarcomere. Titin also contains binding sites for muscle associated proteins so it serves as an adhesion template for the assembly of contractile machinery in muscle cells. It has also been identified as a structural protein for chromosomes. Alternative splicing of this gene results in multiple transcript variants. Considerable variability exists in the I-band, the M-line and the Z-disc regions of titin. Variability in the I-band region contributes to the differences in elasticity of different titin isoforms and, therefore, to the differences in elasticity of different muscle types. Mutations in this gene are associated with familial hypertrophic cardiomyopathy 9, and autoantibodies to titin are produced in patients with the autoimmune disease scleroderma. [provided by RefSeq, Feb 2012]

Function: Key component in the assembly and functioning of vertebrate striated muscles. By providing connections at the level of individual microfilaments, it contributes to the fine balance of forces between the two halves of the sarcomere. The size and extensibility of the cross-links are the main determinants of sarcomere extensibility properties of muscle. In non-muscle cells, seems to play a role in chromosome condensation and chromosome segregation during mitosis. Might link the lamina network to chromatin or nuclear actin, or both during interphase.1 PublicationManual assertion based on experiment in:Ref.28. Catalytic activity: ATP + a protein = ADP + a phosphoprotein. Cofactor: Magnesium. Enzyme regulation: Full activation of the protein kinase domain requires both phosphorylation of Tyr-32341, preventing it from blocking the catalytic aspartate residue, and binding of Ca/CALM to the C-terminal regulatory tail of the molecule which results in ATP binding to the kinase.1 PublicationManual assertion based on experiment in:Ref.28

24-Strip-Wells (LIMIT 1)

240 USD

48-Strip-Wells

495

96-Strip-Wells

710

5x96-Strip-Wells

2705

10x96-Strip-Wells

5145