ELISA/assay

Human 1, 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial, CYP24A1 ELISA Kit

MBS913884

48-Strip-Wells

510 USD

ELISA Kit

Human 1, 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial, CYP24A1 ELISA Kit

1, 25-dihydroxyvitamin D (3) 24-hydroxylase, mitochondrial (CYP24A1) , cytochrome P450, family 24, subfamily A, polypeptide 1

Human 1; 25-dihydroxyvitamin D (3) 24-hydroxylase; mitochondrial (CYP24A1) ELISA kit; CP24; CYP24; MGC126273; MGC126274; P450-CC24; 1; 25-@dihydroxyvitamin D3 24-hydroxylase; 24-OHase; cytochrome P450 family 24 subfamily A polypeptide 1; cytochrome P450; subfamily XXIV (vitamin D 24-hydroxylase) ; exo-mitochondrial; 1; 25-dihydroxyvitamin D (3) 24-hydroxylase; mitochondrial (CYP24A1); cytochrome P450; family 24; subfamily A; polypeptide 1

1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial isoform 1; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 1,25-dihydroxyvitamin D(3) 24-hydroxylase, mitochondrial; 24-OHase; cytochrome P450 24A1; cytochrome P450-CC24; vitamin D 24-hydroxylase; exo-mitochondrial protein; vitamin D(3) 24-hydroxylase; 1,25-@dihydroxyvitamin D3 24-hydroxylase; cytochrome P450, subfamily XXIV (vitamin D 24-hydroxylase); cytochrome P450, family 24, subfamily A, polypeptide 1; Cytochrome P450 24A1; Cytochrome P450-CC24

CYP24A1

CYP24A1; CYP24A1; CP24; HCAI; CYP24; P450-CC24; CYP24; 24-OHase; Vitamin D(3) 24-hydroxylase

CP24A_HUMAN

Human

514

This assay has high sensitivity and excellent specificity for detection of Human CYP24A1. No significant cross-reactivity or interference between Human CYP24A1 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 7.8 pg/ml-500 pg/ml. Sensitivity: 1.95 pg/ml

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CYP24A1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CYP24A1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CYP24A1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CYP24A1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

55770850

NP_000773.2

NM_000782.4

Q07973

58,875 Da

Biological Oxidations Pathway (105698); Cytochrome P450 - Arranged By Substrate Type Pathway (105700); Metabolism Pathway (477135); Metabolism Of Lipids And Lipoproteins Pathway (160976); Metabolism Of Steroid Hormones And Vitamins A And D Pathway (106150); Nuclear Receptors In Lipid Metabolism And Toxicity Pathway (198887); Phase 1 - Functionalization Of Compounds Pathway (105699); Steroid Biosynthesis Pathway (82937); Steroid Biosynthesis Pathway (298); Vitamin D (calciferol) Metabolism Pathway (160956)

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This mitochondrial protein initiates the degradation of 1,25-dihydroxyvitamin D3, the physiologically active form of vitamin D3, by hydroxylation of the side chain. In regulating the level of vitamin D3, this enzyme plays a role in calcium homeostasis and the vitamin D endocrine system. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

CYP24A1: Has a role in maintaining calcium homeostasis. Catalyzes the NADPH-dependent 24-hydroxylation of calcidiol (25- hydroxyvitamin D(3)) and calcitriol (1-alpha,25-dihydroxyvitamin D(3)). The enzyme can perform up to 6 rounds of hydroxylation of calcitriol leading to calcitroic acid. It also shows 23- hydroxylating activity leading to 1-alpha,25-dihydroxyvitamin D(3)-26,23-lactone as end product. Defects in CYP24A1 are the cause of hypercalcemia infantile (HCAI). HCAI is a disorder characterized by abnormally high level of calcium in the blood, failure to thrive, vomiting, dehydration, and nephrocalcinosis. Belongs to the cytochrome P450 family. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Mitochondrial; EC 1.14.13.126; Oxidoreductase. Chromosomal Location of Human Ortholog: 20q13. Cellular Component: mitochondrial inner membrane. Molecular Function: iron ion binding; heme binding; 25-hydroxycholecalciferol-24-hydroxylase activity; oxidoreductase activity; 1-alpha,25-dihydroxyvitamin D3 (1,25-(OH)2D3) 24-hydroxylase activity. Biological Process: osteoblast differentiation; steroid metabolic process; response to vitamin D; vitamin metabolic process; xenobiotic metabolic process; vitamin D metabolic process; vitamin D catabolic process. Disease: Hypercalcemia, Infantile

48-Strip-Wells

510 USD

96-Strip-Wells

725

5x96-Strip-Wells

2565

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4800