ELISA/assay

Human Chitinase-3-like protein 2, CHI3L2 ELISA Kit

MBS913750

96 Strip Wells

760 USD

ELISA Kit

Human Chitinase-3-like protein 2, CHI3L2 ELISA Kit

chitinase 3-like 2

Human Chitinase-3-like protein 2 (CHI3L2) ELISA kit; YKL-39; YKL39; chondrocyte protein 39; chitinase 3-like 2

chitinase-3-like protein 2 isoform b; Chitinase-3-like protein 2; chitinase-3-like protein 2; chondrocyte protein 39; chitinase 3-like 2; Chondrocyte protein 39; YKL-39

CHI3L2

CHI3L2; CHI3L2; CHIL2; YKL39; YKL-39

CH3L2_HUMAN

Human

380

This assay has high sensitivity and excellent specificity for detection of human CHI3L2. No significant cross-reactivity or interference between human CHI3L2 and analogues was observed.

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Samples: For the quantitative determination of human chitinase-3-like protein 2 (CHI3L2) concentrations in serum, plasma, tissue homogenates. Detection Range: 0.312 ng/ml -20 ng/ml.

Sensitivity: The minimum detectable dose of human CHI3L2 is typically less than 0.078 ng/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations. Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHI3L2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHI3L2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHI3L2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHI3L2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

68533253

NP_001020368.1

NM_001025197.1

Q15782

43,501 Da

The protein encoded by this gene is similar to bacterial chitinases but lacks chitinase activity. The encoded protein is secreted and is involved in cartilage biogenesis. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2012]

Function: Lectin that binds chitooligosaccharides and other glycans with high affinity, but not heparin. Has no chitinase activity.1 PublicationManual assertion based on experiment in:Ref.8

96 Strip Wells

760 USD