Mouse Troponin T, cardiac muscle, TNNT2 ELISA Kit | MyBioSource MBS913745 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Mouse Troponin T, cardiac muscle, TNNT2 ELISA Kit

catalog :

MBS913745

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS913745

products type :

ELISA Kit

products full name :

Mouse Troponin T, cardiac muscle, TNNT2 ELISA Kit

products short name :

troponin T type 2 (cardiac)

products name syn :

Mouse Troponin T; cardiac muscle (TNNT2) ELISA kit; CMH2; CMPD2; MGC3889; RCM3; TnTC; cTnT; OTTHUMP00000033864; OTTHUMP00000033867; cardiomyopathy; dilated 1D (autosomal dominant) ; cardiomyopathy; hypertrophic 2; troponin T type 2; cardiac; troponin T; ca; troponin T type 2 (cardiac)

other names :

troponin T, cardiac muscle isoform b; Troponin T, cardiac muscle; troponin T, cardiac muscle; tnTc; cardiac TnT; cardiac muscle troponin T; troponin T2, cardiac; Cardiac muscle troponin T; cTnT

products gene name :

TNNT2

other gene names :

Tnnt2; Tnnt2; Tnt; cTnT; TnTc; cTnT

uniprot entry name :

TNNT2_MOUSE

reactivity :

Mouse

sequence length :

301

specificity :

This assay has high sensitivity and excellent specificity for detection of mouse TNNT2. No significant cross-reactivity or interference between mouse TNNT2 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 31.2 5 pg/ml -2000 pg/ml. Sensitivity: The minimum detectable dose of mouse TNNT2 is typically less than 7.8 pg/ml.The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean O.D value of 20 replicates of the zero standard added by their three standard deviations.

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.

products description :

Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for TNNT2 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TNNT2 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TNNT2 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TNNT2 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

194328745

ncbi acc num :

NP_001123648.1

ncbi gb acc num :

NM_001130176.1

uniprot acc num :

P50752

ncbi mol weight :

35,825 Da

ncbi pathways :

Cardiac Muscle Contraction Pathway (93345); Cardiac Muscle Contraction Pathway (93992); Dilated Cardiomyopathy Pathway (121495); Dilated Cardiomyopathy Pathway (121285); Hypertrophic Cardiomyopathy (HCM) Pathway (114298); Hypertrophic Cardiomyopathy (HCM) Pathway (106591); Muscle Contraction Pathway (574920); Striated Muscle Contraction Pathway (574921); Striated Muscle Contraction Pathway (198348)

uniprot summary :

TNNT2: Troponin T is the tropomyosin-binding subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Heart. The fetal heart shows a greater expression in the atrium than in the ventricle, while the adult heart shows a greater expression in the ventricle than in the atrium. Isoform 6 predominates in normal adult heart. Isoforms 1, 7 and 8 are expressed in fetal heart. Isoform 7 is also expressed in failing adult heart. Belongs to the troponin T family. 11 isoforms of the human protein are produced by alternative splicing. Protein type: Motility/polarity/chemotaxis; Motor. Cellular Component: sarcomere; troponin complex; myofibril; cytoplasm; striated muscle thin filament. Molecular Function: protein binding, bridging; troponin C binding; structural constituent of cytoskeleton; troponin I binding; ATPase activity; tropomyosin binding; actin binding. Biological Process: actin crosslink formation; regulation of muscle contraction; protein heterooligomerization; atrial cardiac muscle morphogenesis; metabolic process; heart development; regulation of muscle filament sliding speed; sarcomere organization; regulation of heart contraction; muscle filament sliding; muscle contraction; positive regulation of ATPase activity; ventricular cardiac muscle morphogenesis; response to calcium ion; negative regulation of ATPase activity; cardiac muscle contraction

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage