catalog number :
MBS913688
products type :
ELISA Kit
products full name :
Pig High mobility group protein B1, HMGB1 ELISA Kit
products short name :
high-mobility group box 1
products name syn :
Pig High mobility group protein B1 (HMGB1) ELISA kit; DKFZp686A04236; HMG1; HMG3; SBP-1; Amphoterin; OTTHUMP00000018196; OTTHUMP00000190860; OTTHUMP00000200117; Sulfoglucuronyl carbohydrate binding protein; high mobility group box 1; high mobility group prot; high-mobility group box 1
other names :
high mobility group protein B1; High mobility group protein B1; high mobility group protein B1; HMG-1; non-histone protein HMG1; high-mobility group box 1; high mobility group protein 1; High mobility group protein 1; HMG-1
products gene name :
HMGB1
other gene names :
HMGB1; HMGB1; HMG1; HMG-1
uniprot entry name :
HMGB1_PIG
specificity :
This assay has high sensitivity and excellent specificity for detection of Pig HMGB1. No significant cross-reactivity or interference between Pig HMGB1 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 0.312 ng/ml-20 ng/ml. Sensitivity: 0.078 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for HMGB1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any HMGB1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for HMGB1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of HMGB1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001004034.1
ncbi gb acc num :
NM_001004034.1
ncbi mol weight :
24,917 Da
ncbi pathways :
Base Excision Repair Pathway (92797); Base Excision Repair Pathway (451)
uniprot summary :
Function: DNA binding proteins that associates with chromatin and has the ability to bend DNA. Binds preferentially single-stranded DNA. Involved in V(D)J recombination by acting as a cofactor of the RAG complex. Acts by stimulating cleavage and RAG protein binding at the 23 bp spacer of conserved recombination signal sequences (RSS). Heparin-binding protein that has a role in the extension of neurite-type cytoplasmic processes in developing cells
size4 :
10x96-Strip-Wells