Human Pancreatic secretory trypsin inhibitor, SPINK1 ELISA Kit | MyBioSource MBS913572 product information

product summary

company name :

MyBioSource

product type :

ELISA/assay

product name :

Human Pancreatic secretory trypsin inhibitor, SPINK1 ELISA Kit

catalog :

MBS913572

quantity :

48-Strip-Wells

price :

510 USD

more info or order :

MyBioSource product webpage

product information

catalog number :

MBS913572

products type :

ELISA Kit

products full name :

Human Pancreatic secretory trypsin inhibitor, SPINK1 ELISA Kit

products short name :

serine peptidase inhibitor, Kazal type 1

products name syn :

Human Pancreatic secretory trypsin inhibitor (SPINK1) ELISA kit; PCTT; PSTI; Spink3; TATI; pancreatic secretory trypsin inhibitor; serine protease inhibitor; Kazal type 1; tumor-associated trypsin inhibitor; serine peptidase inhibitor; Kazal type 1

other names :

pancreatic secretory trypsin inhibitor; Pancreatic secretory trypsin inhibitor; pancreatic secretory trypsin inhibitor; tumor-associated trypsin inhibitor; serine protease inhibitor, Kazal type 1; serine peptidase inhibitor, Kazal type 1; Serine protease inhibitor Kazal-type 1; Tumor-associated trypsin inhibitor; TATI

products gene name :

SPINK1

other gene names :

SPINK1; SPINK1; TCP; PCTT; PSTI; TATI; Spink3; PSTI; TATI

uniprot entry name :

ISK1_HUMAN

reactivity :

Human

sequence length :

specificity :

This assay has high sensitivity and excellent specificity for detection of Human SPINK1. No significant cross-reactivity or interference between Human SPINK1 and analogues was observed.

storage stability :

Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

other info1 :

Samples: Serum, plasma, urine, cell culture supernates. Assay Type: Sandwich. Detection Range: 3.12 ng/ml-200 ng/ml. Sensitivity: 0.78 ng/ml

other info2 :

Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul

products description :

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPINK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPINK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPINK1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPINK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

ncbi gi num :

45505132

ncbi acc num :

NP_003113.2

ncbi gb acc num :

NM_003122.4

uniprot acc num :

P00995

ncbi mol weight :

8,507 Da

ncbi summary :

The protein encoded by this gene is a trypsin inhibitor, which is secreted from pancreatic acinar cells into pancreatic juice. It is thought to function in the prevention of trypsin-catalyzed premature activation of zymogens within the pancreas and the pancreatic duct. Mutations in this gene are associated with hereditary pancreatitis and tropical calcific pancreatitis. [provided by RefSeq, Oct 2008]

uniprot summary :

SPINK1: This is a trypsin inhibitor, its physiological function is to prevent the trypsin-catalyzed premature activation of zymogens within the pancreas. Defects in SPINK1 are a cause of pancreatitis (PCTT). A disease characterized by the presence of calculi in pancreatic ducts. It causes severe abdominal pain attacks. Defects in SPINK1 are the cause of susceptibility to tropical calcific pancreatitis (TCP). TCP is an idiopathic, juvenile, nonalcoholic form of chronic pancreatitis widely prevalent in several tropical countries. It can be associated with fibrocalculous pancreatic diabetes (FCPD) depending on both environmental and genetic factors. TCP differs from alcoholic pancreatitis by a much younger age of onset, pancreatic calcification, a high incidence of insulin dependent but ketosis resistant diabetes mellitus, and an exceptionally high incidence of pancreatic cancer. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 5q32. Cellular Component: extracellular space. Molecular Function: serine-type endopeptidase inhibitor activity; protein binding; endopeptidase inhibitor activity. Biological Process: negative regulation of peptidyl-tyrosine phosphorylation; regulation of acrosome reaction. Disease: Tropical Calcific Pancreatitis; Pancreatitis, Hereditary

size1 :

48-Strip-Wells

price1 :

510 USD

size2 :

96-Strip-Wells

price2 :

725

size3 :

5x96-Strip-Wells

price3 :

2565

size4 :

10x96-Strip-Wells

price4 :

4800

more info or order :

MyBioSource product webpage