catalog number :
MBS913550
products type :
ELISA Kit
products full name :
Human secretogranin-1 (chromogranin-B) , CHGB/SCG1 ELISA Kit
products short name :
chromogranin B (secretogranin 1)
products name syn :
Human secretogranin-1 (chromogranin-B) (CHGB/SCG1) ELISA kit; SCG1; chromogranin B; secretogranin B; chromogranin B (secretogranin 1)
other names :
secretogranin-1; Secretogranin-1; secretogranin-1; cgB; sgI; chromogranin-B; secretogranin I; Chromogranin-B; CgB; Secretogranin I; SgI
products gene name :
CHGB
other gene names :
CHGB; CHGB; CgB; SgI
uniprot entry name :
SCG1_BOVIN
specificity :
This assay has high sensitivity and excellent specificity for detection of Human CHGB. No significant cross-reactivity or interference between Human CHGB and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates. Assay Type: Sandwich. Detection Range: 6.25 ng/ml-400 ng/ml. Sensitivity: 1.56 ng/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% is less than 10%. Three samples of known concentration were tested in twenty assays to assess. Detection Wavelength: 450 nm. Sample Volume: 50-100ul
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for CHGB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CHGB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CHGB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CHGB bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_851349.1
ncbi gb acc num :
NM_181006.2
ncbi mol weight :
73,340 Da
uniprot summary :
Function: Secretogranin-1 is a neuroendocrine secretory granule protein, which may be the precursor for other biologically active peptides. The 16 pairs of basic AA distributed throughout its sequence may be used as proteolytic cleavage sites.
size4 :
10x96-Strip-Wells