catalog number :
MBS913372
products type :
ELISA Kit
products full name :
Human Leucine-rich repeat-containing protein 17, LRRC17 ELISA Kit
products short name :
leucine rich repeat containing 17
products name syn :
Human Leucine-rich repeat-containing protein 17 (LRRC17) ELISA kit; P37NB; 37 kDa leucine-rich repeat (LRR) protein; leucine rich repeat containing 17
other names :
leucine-rich repeat-containing protein 17 isoform 1; Leucine-rich repeat-containing protein 17; leucine-rich repeat-containing protein 17; 37 kDa leucine-rich repeat (LRR) protein; leucine rich repeat containing 17; p37NB
products gene name :
LRRC17
other gene names :
LRRC17; LRRC17; P37NB; P37NB; UNQ3076/PRO9909
uniprot entry name :
LRC17_HUMAN
specificity :
This assay has high sensitivity and excellent specificity for detection of human LRRC17. No significant cross-reactivity or interference between human LRRC17 and analogues was observed.
storage stability :
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.
other info1 :
Samples: Serum, plasma, tissue homogenates, cell lysates. Assay Type: Sandwich. Detection Range: 23.44 pg/ml-1500 pg/ml. Sensitivity: 5.86 pg/ml
other info2 :
Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
products description :
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for LRRC17 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any LRRC17 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for LRRC17 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of LRRC17 bound in the initial step. The color development is stopped and the intensity of the color is measured.
ncbi acc num :
NP_001026862.1
ncbi gb acc num :
NM_001031692.2
ncbi mol weight :
51,800 Da
uniprot summary :
LRRC17: Involved in bone homeostasis. Acts as a negative regulator of RANKL-induced osteoclast precursor differentiation from bone marrow precursors. 2 isoforms of the human protein are produced by alternative splicing. Protein type: Secreted; Secreted, signal peptide. Chromosomal Location of Human Ortholog: 7q22.1. Cellular Component: extracellular space. Biological Process: bone marrow development; osteoblast differentiation; negative regulation of osteoclast differentiation; osteoblast proliferation
size4 :
10x96-Strip-Wells